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A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant

A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant

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PARAMESWARAN, Aravind, Barbara LEITENMAIER, Mingjie YANG, Peter KRONECK, Wolfram WELTE, Gabriela LUTZ, Ashot PAPOYAN, Leon V. KOCHIAN, Hendrik KÜPPER, 2007. A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant. In: Biochemical and Biophysical Research Communications. 363(1), pp. 51-56. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2007.08.105

@article{Parameswaran2007-11-09nativ-15523, title={A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant}, year={2007}, doi={10.1016/j.bbrc.2007.08.105}, number={1}, volume={363}, issn={0006-291X}, journal={Biochemical and Biophysical Research Communications}, pages={51--56}, author={Parameswaran, Aravind and Leitenmaier, Barbara and Yang, Mingjie and Kroneck, Peter and Welte, Wolfram and Lutz, Gabriela and Papoyan, Ashot and Kochian, Leon V. and Küpper, Hendrik} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/15523"> <dc:contributor>Yang, Mingjie</dc:contributor> <dcterms:bibliographicCitation>Publ. in: Biochemical and biophysical research communications ; 363 (2007), 1. - S. 51-56</dcterms:bibliographicCitation> <dc:creator>Kroneck, Peter</dc:creator> <dc:creator>Leitenmaier, Barbara</dc:creator> <dcterms:rights rdf:resource="https://kops.uni-konstanz.de/page/termsofuse"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Lutz, Gabriela</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-12-20T09:29:10Z</dc:date> <dc:creator>Yang, Mingjie</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Parameswaran, Aravind</dc:contributor> <dc:creator>Küpper, Hendrik</dc:creator> <dcterms:abstract xml:lang="eng">We report here the first purification of a P1B type ATPase, a group of transporters that occurs in bacteria, plants and animals incl. humans, from a eukaryotic organism in native state. TcHMA4 is a P1B type ATPase that is highly expressed in the Cd/Zn hyperaccumulator plant Thlaspi caerulescens and contains a C-terminal 9-histidine repeat. After isolation from roots, we purified TcHMA4 protein via metal affinity chromatography. The purified protein exhibited Cd- and Zn-activated ATPase activity after reconstitution into lipid vesicles, showing that it was in its native state. Gels of crude root extract and of the purified protein revealed TcHMA4-specific bands of about 50 and 60 kDa, respectively, while the TcHMA4 mRNA predicts a single protein with a size of 128 kDa. This indicates the occurrence of post-translational processing; the properties of the two bands were characterised by their activity and binding properties.</dcterms:abstract> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Kochian, Leon V.</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Kroneck, Peter</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-12-20T09:29:10Z</dcterms:available> <dc:contributor>Welte, Wolfram</dc:contributor> <dc:contributor>Leitenmaier, Barbara</dc:contributor> <dc:creator>Parameswaran, Aravind</dc:creator> <dc:contributor>Küpper, Hendrik</dc:contributor> <dc:contributor>Papoyan, Ashot</dc:contributor> <dcterms:title>A native Zn/Cd pumping P1B ATPase from natural overexpression in a hyperaccumulator plant</dcterms:title> <dcterms:issued>2007-11-09</dcterms:issued> <dc:creator>Papoyan, Ashot</dc:creator> <dc:creator>Kochian, Leon V.</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/15523"/> <dc:creator>Welte, Wolfram</dc:creator> <dc:creator>Lutz, Gabriela</dc:creator> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

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