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The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233 : characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine

The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233 : characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine

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HUANG, Fanglu, Stephen F. HAYDOCK, Tatiana MIRONENKO, Dieter SPITELLER, Yanyan LI, Jonathan B. SPENCER, 2005. The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233 : characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine. In: Organic & Biomolecular Chemistry. 3(8), pp. 1410-1418. ISSN 1477-0520

@article{Huang2005-04-21neomy-15515, title={The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233 : characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine}, year={2005}, doi={10.1039/B501199J}, number={8}, volume={3}, issn={1477-0520}, journal={Organic & Biomolecular Chemistry}, pages={1410--1418}, author={Huang, Fanglu and Haydock, Stephen F. and Mironenko, Tatiana and Spiteller, Dieter and Li, Yanyan and Spencer, Jonathan B.} }

Publ. in: Organic & biomolecular chemistry ; 3 (2005), 8. - pp. 1410-1418 Spencer, Jonathan B. 2011-09-01T12:18:19Z Mironenko, Tatiana Li, Yanyan 2011-09-01T12:18:19Z Huang, Fanglu Spencer, Jonathan B. Mironenko, Tatiana Haydock, Stephen F. Haydock, Stephen F. deposit-license Spiteller, Dieter eng Huang, Fanglu The biosynthetic gene cluster of the 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotic neomycin has been cloned for the first time by screening of a cosmid library of Streptomyces fradiae NCIMB 8233. Sequence analysis has identified 21 putative open reading frames (ORFs) in the neomycin gene cluster (neo) with significant protein sequence similarity to gene products involved in the biosynthesis of other DOS-containing aminoglycosides, namely butirosin (btr), gentamycin (gnt), tobramycin (tbm) and kanamycin (kan). Located at the 5′-end of the neo gene cluster is the previously-characterised neomycin phosphotransferase gene (apH). Three genes unique to the neo and btr clusters have been revealed by comparison of the neo cluster to btr, gnt, tbm and kan clusters. This suggests that these three genes may be involved in the transfer of a ribose moiety to the DOS ring during the antibiotic biosynthesis. The product of the neo-6 gene is characterised here as the L-glutamine : 2-deoxy-scyllo-inosose aminotransferase responsible for the first transamination in DOS biosynthesis, which supports the assignment of the gene cluster. Spiteller, Dieter The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233 : characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine Li, Yanyan 2005-04-21

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