Monitoring E. amylovora using Real Time PCR

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VÖGELE, Ralf, Stefan KUNZ, L. OLBRECHT, Malin HINZE, S. WEISSHAUPT, A. SCHMID, M. ERNST, M. JOOS, M. MATSCHINSKY, Kurt MENDGEN, 2010. Monitoring E. amylovora using Real Time PCR. Eco-Fruit. University of Hohenheim, Germany, 22. Feb 2010 - 24. Feb 2010. In: FÖRDERGEMEINSCHAFT ÖKOLOGISCHER OBSTBAU E. V., , ed.. Eco-Fruit : proceedings to the conference from February 22 nd to February 24 th, 2010 at the University of Hohenheim, Germany ; 14 th International Conference on Organic Fruit-Growing. Eco-Fruit. University of Hohenheim, Germany, 22. Feb 2010 - 24. Feb 2010. Weinsberg:Fördergemeinschaft Ökologischer Obstbau, pp. 110-117. ISBN 978-3-00-030214-5

@inproceedings{Vogele2010Monit-15162, title={Monitoring E. amylovora using Real Time PCR}, year={2010}, isbn={978-3-00-030214-5}, address={Weinsberg}, publisher={Fördergemeinschaft Ökologischer Obstbau}, booktitle={Eco-Fruit : proceedings to the conference from February 22 nd to February 24 th, 2010 at the University of Hohenheim, Germany ; 14 th International Conference on Organic Fruit-Growing}, pages={110--117}, editor={Fördergemeinschaft Ökologischer Obstbau e. V.}, author={Vögele, Ralf and Kunz, Stefan and Olbrecht, L. and Hinze, Malin and Weißhaupt, S. and Schmid, A. and Ernst, M. and Joos, M. and Matschinsky, M. and Mendgen, Kurt} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/15162"> <dcterms:abstract xml:lang="eng">Fire blight, caused by the Gram-negative bacterium Erwinia amylovora is the most damaging bacterial disease of pome fruit world wide. Disease outbreak is only sporadic, disease outcome, however, can be devastating. Aside from physical factors like temperature and humidity, presence of the pathogen in sufficient amounts to cause infection is a crucial aspect in abatement of disease. We have refined a real time PCR based quantification of E. amylovora to be used in the field on a variety of environmental samples. This method allowed us to monitor pathogen abundance during blossom during the last four years. Integration of the amount of pathogen detected into refined prediction algorithms will most certainly improve applied forecasting models. The method also allows the quantification of pathogen outside the blooming period within or on the surface of different tissue, or plant organs. This may allow a monitoring of the pathogen population throughout the year and in the long run may facilitate timely countermeasures. Results also indicate a potential role of fruit mummies in overwintering and dissemination of the pathogen early in the growing season, as well as a role of non-host flowers in the built up of pathogen potential. The method is also capable of monitoring disease progression in infected, but symptomless tissue. Results from this set of experiments might improve recommendations for good cultural practice.</dcterms:abstract> <dcterms:bibliographicCitation>Publ. in: Eco-Fruit : proceedings to the conference from February 22nd to February 24th, 2010 at the University of Hohenheim, Germany ; 14th International Conference on Organic Fruit-Growing / ed. by Fördergemeinschaft Ökologischer Obstbau e.V. Weinsberg : Fördergemeinschaft Ökologischer Obstbau, 2010. pp. 110-117</dcterms:bibliographicCitation> <dc:rights>deposit-license</dc:rights> <dc:contributor>Schmid, A.</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/15162"/> <dc:creator>Mendgen, Kurt</dc:creator> <dc:creator>Matschinsky, M.</dc:creator> <dc:contributor>Kunz, Stefan</dc:contributor> <dc:creator>Joos, M.</dc:creator> <dc:creator>Vögele, Ralf</dc:creator> <dc:creator>Kunz, Stefan</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-17T08:25:59Z</dcterms:available> <dcterms:issued>2010</dcterms:issued> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-17T08:25:59Z</dc:date> <dc:contributor>Matschinsky, M.</dc:contributor> <dc:contributor>Joos, M.</dc:contributor> <dc:contributor>Olbrecht, L.</dc:contributor> <dc:contributor>Hinze, Malin</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:contributor>Weißhaupt, S.</dc:contributor> <dc:contributor>Ernst, M.</dc:contributor> <dc:creator>Ernst, M.</dc:creator> <dc:creator>Hinze, Malin</dc:creator> <dc:contributor>Mendgen, Kurt</dc:contributor> <dc:contributor>Vögele, Ralf</dc:contributor> <dc:creator>Schmid, A.</dc:creator> <dcterms:title>Monitoring E. amylovora using Real Time PCR</dcterms:title> <dc:creator>Weißhaupt, S.</dc:creator> <dc:language>eng</dc:language> <dc:creator>Olbrecht, L.</dc:creator> </rdf:Description> </rdf:RDF>

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