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Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect

Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect

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SCHRACKE, Nadine, Tobias KORNMEYER, Marcel KRÄNZLE, Peter F. STÄHLER, Daniel SUMMERER, Markus BEIER, 2009. Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect. In: New Biotechnology. 26(5), pp. 229-233. ISSN 1871-6784. eISSN 1876-4347

@article{Schracke2009-11-30Speci-14965, title={Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect}, year={2009}, doi={10.1016/j.nbt.2009.08.013}, number={5}, volume={26}, issn={1871-6784}, journal={New Biotechnology}, pages={229--233}, author={Schracke, Nadine and Kornmeyer, Tobias and Kränzle, Marcel and Stähler, Peter F. and Summerer, Daniel and Beier, Markus} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/14965"> <dc:contributor>Kornmeyer, Tobias</dc:contributor> <dc:contributor>Beier, Markus</dc:contributor> <dc:contributor>Kränzle, Marcel</dc:contributor> <dc:creator>Summerer, Daniel</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-19T12:52:57Z</dcterms:available> <dcterms:issued>2009-11-30</dcterms:issued> <dcterms:abstract xml:lang="eng">Recent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect(TM), a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-19T12:52:57Z</dc:date> <dc:creator>Schracke, Nadine</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Schracke, Nadine</dc:contributor> <dc:contributor>Summerer, Daniel</dc:contributor> <dc:creator>Stähler, Peter F.</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:contributor>Stähler, Peter F.</dc:contributor> <dcterms:bibliographicCitation>Publ. in: New Biotechnology ; 26 (2009), 5. - pp. 229-233</dcterms:bibliographicCitation> <dc:creator>Beier, Markus</dc:creator> <dcterms:title>Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect</dcterms:title> <dc:creator>Kränzle, Marcel</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14965"/> <dc:creator>Kornmeyer, Tobias</dc:creator> </rdf:Description> </rdf:RDF>

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