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Dissection of the xeroderma pigmentosum group C protein function by sitedirected mutagenesis

Dissection of the xeroderma pigmentosum group C protein function by sitedirected mutagenesis

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CLEMENT, Flurina C., Nina KACZMAREK, Nadine MATHIEU, Martin TOMAS, Alfred LEITENSTORFER, Elisa MAY, Hanspeter NAEGELI, 2011. Dissection of the xeroderma pigmentosum group C protein function by sitedirected mutagenesis. In: Antioxidants & Redox Signaling. 14(12), pp. 2479-2490. ISSN 1523-0864. eISSN 1557-7716

@article{Clement2011-06-15Disse-14811, title={Dissection of the xeroderma pigmentosum group C protein function by sitedirected mutagenesis}, year={2011}, doi={10.1089/ars.2010.3399}, number={12}, volume={14}, issn={1523-0864}, journal={Antioxidants & Redox Signaling}, pages={2479--2490}, author={Clement, Flurina C. and Kaczmarek, Nina and Mathieu, Nadine and Tomas, Martin and Leitenstorfer, Alfred and May, Elisa and Naegeli, Hanspeter} }

eng Mathieu, Nadine 2011-06-15 Clement, Flurina C. Tomas, Martin Leitenstorfer, Alfred Firs publ. in: Antioxidants & Redox Signaling ; 14 (2011), 12. - pp. 2479-2490 May, Elisa deposit-license Kaczmarek, Nina Leitenstorfer, Alfred Naegeli, Hanspeter Xeroderma pigmentosum group C (XPC) protein is a sensor of helix-distorting DNA lesions, the function of which is to trigger the global genome repair (GGR) pathway. Previous studies demonstrated that XPC protein operates by detecting the single-stranded character of non-hydrogen-bonded bases opposing lesion sites. This mode of action is supported by structural analyses of the yeast Rad4 homologue that identified critical side chains making close contacts with a pair of extrahelical nucleotides. Here, alanine substitutions of the respective conserved residues (N754, F756, F797, F799) in human XPC were tested for DNA-binding activity, accumulation in tracks and foci of DNA lesions, nuclear protein mobility, and the induction of downstream GGR reactions. This study discloses a dynamic interplay between XPC protein and DNA, whereby the association with one displaced nucleotide in the undamaged strand mediates the initial encounter with lesion sites. The additional flipping-out of an adjacent nucleotide is necessary to hand over the damaged site to the next GGR player. Surprisingly, this mutagenesis analysis also reveals that the rapid intranuclear trafficking of XPC protein depends on constitutive interactions with native DNA, implying that the search for base damage takes place in living cells by a facilitated diffusion process. Kaczmarek, Nina Naegeli, Hanspeter Dissection of the xeroderma pigmentosum group C protein function by sitedirected mutagenesis May, Elisa 2011-08-18T12:18:55Z Mathieu, Nadine Tomas, Martin Clement, Flurina C. 2011-08-18T12:18:55Z

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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