Activation of human basophils through the interleukin-8 receptor

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KRIEGER, Martin, Thomas BRUNNER, Stephan BISCHOFF, Vinzenz von TSCHARNER, Alfred WALZ, Bernhard MOSER, Marco BAGGIOLINI, Clemens DAHINDEN, 1992. Activation of human basophils through the interleukin-8 receptor. In: Journal of Immunology. 149(8), pp. 2662-2667. ISSN 0022-1767. eISSN 1550-6606

@article{Krieger1992Activ-14341, title={Activation of human basophils through the interleukin-8 receptor}, year={1992}, number={8}, volume={149}, issn={0022-1767}, journal={Journal of Immunology}, pages={2662--2667}, author={Krieger, Martin and Brunner, Thomas and Bischoff, Stephan and Tscharner, Vinzenz von and Walz, Alfred and Moser, Bernhard and Baggiolini, Marco and Dahinden, Clemens} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:creator>Dahinden, Clemens</dc:creator> <dc:contributor>Dahinden, Clemens</dc:contributor> <bibo:uri rdf:resource=""/> <dc:creator>Krieger, Martin</dc:creator> <dc:creator>Baggiolini, Marco</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:date rdf:datatype="">2011-10-25T06:58:30Z</dc:date> <dc:creator>Tscharner, Vinzenz von</dc:creator> <dc:creator>Brunner, Thomas</dc:creator> <dc:contributor>Moser, Bernhard</dc:contributor> <dc:language>eng</dc:language> <dcterms:issued>1992</dcterms:issued> <dc:contributor>Krieger, Martin</dc:contributor> <dspace:isPartOfCollection rdf:resource=""/> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:contributor>Baggiolini, Marco</dc:contributor> <dc:contributor>Walz, Alfred</dc:contributor> <dcterms:isPartOf rdf:resource=""/> <dcterms:abstract xml:lang="eng">IL-8 and its structural analogs derived from blood platelets have been proposed as stimuli of IgE-independent basophil activation. In order to clarify the mechanism of action of these peptides, we examined the effects of pure IL-8, connective tissue-activating peptide III (CTAP- III), neutrophil-activating peptide 2 (NAP-2), and platelet factor 4 (PF-4) on blood basophils with and without pretreatment by IL-3, which modulates mediator release. After pretreatment with IL-3, significant histamine release was observed with 10(-8) M and 10(-7) M IL-8 and 10(- 7) M NAP-2, but not with the other peptides. At higher concentrations (10(-6) M), however, all IL-8 analogs, as well as the unrelated cationic peptides poly-D-lysine, histone VS, and lysozyme, induced histamine release to variable degrees. Binding and competition studies with [125I]IL-8 revealed specific IL-8R on basophils from a patient with chronic myelogenous leukemia and normal individuals. From 3500 to 9600 receptors with a mean Kd value of 0.15 nM were found on average per chronic myelogenous leukemia and normal basophil, respectively. NAP- 2 weakly competed for IL-8 binding. IL-8 and, to a lesser extent, NAP-2 led to a transient rise of cytosolic free calcium concentration ([Ca2+]i), which was independent of a preexposure to IL-3. IL-8 prevented the [Ca2+]i rise induced by NAP-2, but did not influence [Ca2+]i responses to other agonists, e.g. C5a, C3a, or platelet- activating factor. IL-8 induced [Ca2+]i changes and histamine release in IL-3-primed basophils were pertussis toxin sensitive. CTAP-III or PF- 4 did not compete for IL-8 binding, did not induce [Ca2+]i changes, and did not influence the [Ca2+]i response to IL-8 and NAP-2. This study shows that IL-8 and NAP-2 activate human basophils by a receptor- mediated mechanism similar to that operating in neutrophils. At high concentrations histamine release can also be induced by cationic peptides by a mechanism that does not involve the IL-8R, and probably depends on cationic interactions.</dcterms:abstract> <dc:creator>Moser, Bernhard</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Bischoff, Stephan</dc:creator> <dcterms:available rdf:datatype="">2011-10-25T06:58:30Z</dcterms:available> <dcterms:title>Activation of human basophils through the interleukin-8 receptor</dcterms:title> <dcterms:bibliographicCitation>First publ. in: Journal of Immunology ; 149 (1992), 8. - pp. 2662-2667</dcterms:bibliographicCitation> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Bischoff, Stephan</dc:contributor> <dcterms:rights rdf:resource=""/> <dc:creator>Walz, Alfred</dc:creator> <dc:contributor>Tscharner, Vinzenz von</dc:contributor> </rdf:Description> </rdf:RDF>

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