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RANTES and macrophage inflammatory protein 1 alpha induce the migration and activation of normal human eosinophil granulocytes

RANTES and macrophage inflammatory protein 1 alpha induce the migration and activation of normal human eosinophil granulocytes

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ROT, Antal, Martin KRIEGER, Thomas BRUNNER, Stephan BISCHOFF, Thomas SCHALL, Clemens DAHINDEN, 1992. RANTES and macrophage inflammatory protein 1 alpha induce the migration and activation of normal human eosinophil granulocytes. In: Journal of Experimental Medicine. 176(6), pp. 1489-1495. ISSN 0022-1007

@article{Rot1992RANTE-14340, title={RANTES and macrophage inflammatory protein 1 alpha induce the migration and activation of normal human eosinophil granulocytes}, year={1992}, doi={10.1084/jem.176.6.1489}, number={6}, volume={176}, issn={0022-1007}, journal={Journal of Experimental Medicine}, pages={1489--1495}, author={Rot, Antal and Krieger, Martin and Brunner, Thomas and Bischoff, Stephan and Schall, Thomas and Dahinden, Clemens} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/14340"> <dcterms:issued>1992</dcterms:issued> <dc:contributor>Krieger, Martin</dc:contributor> <dc:creator>Bischoff, Stephan</dc:creator> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:contributor>Bischoff, Stephan</dc:contributor> <dcterms:abstract xml:lang="eng">The cellular infiltrates of certain inflammatory processes found in parasitic infection or in allergic diseases consist predominantly of eosinophilic granulocytes, often in association with activated T cells. This suggests the existence of chemotactic agonists specific for eosinophils and lymphocyte subsets devoid of neutrophil-activating properties. We therefore examined four members of the intercrine/chemokine superfamily of cytokines (monocyte chemotactic peptide 1 [MCP-1], RANTES, macrophage inflammatory protein 1 alpha [MIP-1 alpha], and MIP-1 beta), which do not activate neutrophils, for their ability to affect different eosinophil effector functions. RANTES strongly attracted normal human eosinophils by a chemotactic rather than a chemokinetic mechanism with a similar efficacy as the most potent chemotactic myeloid cell agonist, C5a. MIP-1 alpha also induced eosinophil migration, however, with lower efficacy. RANTES and MIP-1 alpha induced eosinophil cationic protein release in cytochalasin B-treated eosinophils, but did not promote leukotriene C4 formation by eosinophils, even after preincubation with interleukin 3 (IL-3), in contrast to other chemotactic agonists such as C5a and formyl-methionyl-leucyl-phenylalanine (FMLP). RANTES, but not MIP-1 alpha, induced a biphasic chemiluminescence response, however, of lower magnitude than C5a. RANTES and MIP-1 alpha both promoted identical transient changes in intracellular free calcium concentration ([Ca2+]i), with kinetics similar to those induced by chemotactic peptides known to interact with G protein-coupled receptors. No cross-desensitization towards other peptide agonists (e.g., C5a, IL-8, FMLP) was observed, suggesting the presence of specific receptors. Despite its weaker eosinophil-activating properties, MIP-1 alpha was at least 10 times more potent on a molar basis than RANTES at inducing [Ca2+]i changes. Interestingly, RANTES deactivated the MIP-1 alpha-induced [Ca2+]i changes, while the RANTES response was preserved after MIP-1 alpha stimulation. MCP-1, a potent monocyte chemoattractant and basophil agonist, as well as MIP-1 beta, a peptide with pronounced homology to MIP-1 alpha, did not activate the eosinophil functions tested. Our results indicate that RANTES and MIP-1 alpha are crucial mediators of inflammatory processes in which eosinophils predominate.</dcterms:abstract> <dc:creator>Rot, Antal</dc:creator> <dc:creator>Dahinden, Clemens</dc:creator> <dc:contributor>Rot, Antal</dc:contributor> <dc:contributor>Dahinden, Clemens</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:contributor>Schall, Thomas</dc:contributor> <dcterms:title>RANTES and macrophage inflammatory protein 1 alpha induce the migration and activation of normal human eosinophil granulocytes</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-25T06:31:23Z</dcterms:available> <dc:rights>deposit-license</dc:rights> <dcterms:bibliographicCitation>First publ. in: Journal of Experimental Medicine ; 176 (1992), 6. - pp. 1489-1495</dcterms:bibliographicCitation> <dc:creator>Krieger, Martin</dc:creator> <dc:creator>Schall, Thomas</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14340"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-25T06:31:23Z</dc:date> <dc:creator>Brunner, Thomas</dc:creator> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

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