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Human peripheral blood basophils primed by IL-3 produce IL-4 in response to IgE receptor stimulation

Human peripheral blood basophils primed by IL-3 produce IL-4 in response to IgE receptor stimulation

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BRUNNER, Thomas, Christoph HEUSSER, Clemens DAHINDEN, 1993. Human peripheral blood basophils primed by IL-3 produce IL-4 in response to IgE receptor stimulation. In: Journal of Experimental Medicine. 177(3), pp. 605-611. ISSN 0022-1007. Available under: doi: 10.1084/jem.177.3.605

@article{Brunner1993Human-14338, title={Human peripheral blood basophils primed by IL-3 produce IL-4 in response to IgE receptor stimulation}, year={1993}, doi={10.1084/jem.177.3.605}, number={3}, volume={177}, issn={0022-1007}, journal={Journal of Experimental Medicine}, pages={605--611}, author={Brunner, Thomas and Heusser, Christoph and Dahinden, Clemens} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/14338"> <dcterms:bibliographicCitation>First publ. in: Journal of Experimental Medicine ; 177 (1993), 3. - pp. 605-611</dcterms:bibliographicCitation> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14338/1/1993_Brunner_JEM_93_177_605.pdf"/> <dc:creator>Dahinden, Clemens</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-25T06:01:55Z</dc:date> <dc:contributor>Dahinden, Clemens</dc:contributor> <dc:creator>Heusser, Christoph</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14338"/> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14338/1/1993_Brunner_JEM_93_177_605.pdf"/> <dc:contributor>Heusser, Christoph</dc:contributor> <dcterms:abstract xml:lang="eng">In contrast to most cytokines, interleukin 4 (IL-4) expression is restricted to T lymphocytes, with the exception of mast cell lines and mast cells, as more recently demonstrated in rodents. Little is known, however, about the capacity of human nonlymphoid cells to produce IL-4. In this study we show that mature human basophils are capable of expressing IL-4 and examine the regulation of IL-4 production in comparison with the lipid mediator leukotriene C4. IL-4 was produced upon immunoglobulin E receptor (IgER) activation of basophils cultured with IL-3, a cytokine previously shown to prime these cells for enhanced release of inflammatory mediators. In some experiments, IL-3 or IgER activation alone also induced IL-4 production close to the detection limit. The effect of IL-3 on IgER-dependent IL-4 expression was dose and time dependent: maximal IL-4 production occurred between 18 and 48 h preexposure of basophils to 3-10 ng/ml IL-3. IgER-induced IL-4 synthesis and release by basophils cultured with IL-3 was rapid and complete after 6 h. In contrast to IL-3, other cytokines (IL-5, granulocyte/macrophage colony-stimulating factor, and nerve growth factor) that also prime basophils for enhanced histamine and leukotriene C4 release did not promote IgER-induced IL-4 synthesis. Basophils appear to secrete a "TH2-like" cytokine profile since no detectable IL-2 or interferon gamma was produced upon IgER activation. Mononuclear cells (depleted of basophils), cultured in parallel, did not release IL-4 in response to IL-3 and/or IgER activation, and produced approximately ten times less IL-4 than basophils upon nonspecific activation by phorbol ester and calcium ionophore. Thus, human basophils are an important cellular source of IL-4, and may, therefore, in addition to their inflammatory effector functions, also regulate the differentiation of T helper cells and B cells, in particular in allergic diseases.</dcterms:abstract> <dcterms:title>Human peripheral blood basophils primed by IL-3 produce IL-4 in response to IgE receptor stimulation</dcterms:title> <dcterms:issued>1993</dcterms:issued> <dc:creator>Brunner, Thomas</dc:creator> <dc:rights>terms-of-use</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-25T06:01:55Z</dcterms:available> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:language>eng</dc:language> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> </rdf:Description> </rdf:RDF>

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