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Immunolocalization of intracellular interleukin-4 in normal human peripheral blood basophils

Immunolocalization of intracellular interleukin-4 in normal human peripheral blood basophils

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MUELLER, Regula, Christoph H. HEUSSER, Silvia RIHS, Thomas BRUNNER, Gillian R. BULLOCK, Clemens A. DAHINDEN, 1994. Immunolocalization of intracellular interleukin-4 in normal human peripheral blood basophils. In: European Journal of Immunology. 24(12), pp. 2935-2940. ISSN 0014-2980. Available under: doi: 10.1002/eji.1830241203

@article{Mueller1994-12Immun-14329, title={Immunolocalization of intracellular interleukin-4 in normal human peripheral blood basophils}, year={1994}, doi={10.1002/eji.1830241203}, number={12}, volume={24}, issn={0014-2980}, journal={European Journal of Immunology}, pages={2935--2940}, author={Mueller, Regula and Heusser, Christoph H. and Rihs, Silvia and Brunner, Thomas and Bullock, Gillian R. and Dahinden, Clemens A.} }

Rihs, Silvia Dahinden, Clemens A. Rihs, Silvia 2011-08-05T08:12:30Z Brunner, Thomas Bullock, Gillian R. 2011-08-05T08:12:30Z deposit-license The question as to whether other cell types apart from helper T lymphocytes are capable of producing interleukin-4 (IL-4) has gained much interest over the last years. Recent studies indicate that human basophils also produce IL-4, although direct proof is missing so far. In this study we demonstrate the presence of IL-4 in the cytoplasm of in vitro activated human peripheral blood basophils derived from normal donors. Cytokine-producing cells were revealed at the single-cell level by intracellular immunofluorescence staining using IL-4-specific monoclonal antibodies. Basophils showed a characteristic, apparently granular staining pattern easily discerned from the eccentric dot-shaped staining pattern in activated T cells used in control experiments. Cell counts following priming with IL-3 and stimulation with polyclonal sheep anti-IgE antibody or the anaphylatoxin C5a revealed a significant increase in IL-4-positive basophils to about 19% as compared with unprimed, unstimulated control cells (6%). The amount of IL-4 in the supernatant of these cell preparations paralleled these observations with an at least five- to sevenfold increase following stimulation as compared with control cells (< 5 ng/ml). Using confocal scanning laser microscopy, the intracellular presence of IL-4 was confirmed, and the cells were identified as being basophils on terms of their characteristic multilobed nucleus. This observation was supported by double labeling studies using antibodies to IL-4 and to the high-affinity IgE receptor (FcεR1). Interestingly, stimulation of cells led to a decrease in the number of FcεR1-positive cells. The above results show direct evidence that IL-4 is produced by activated human basophils. Heusser, Christoph H. Mueller, Regula 1994-12 Immunolocalization of intracellular interleukin-4 in normal human peripheral blood basophils eng Heusser, Christoph H. Brunner, Thomas Publ. in: European Journal of Immunology ; 24 (1994), 12. - pp. 2935-2940 Mueller, Regula Bullock, Gillian R. Dahinden, Clemens A.

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