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Immunoregulatory activity of a T cell receptor alpha chain demonstrated by in vitro transcription and translation

Immunoregulatory activity of a T cell receptor alpha chain demonstrated by in vitro transcription and translation

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Prüfsumme: MD5:424ea28b8575f0053ae8de44a6fbeab7

ONDA, Takebumi, Thomas BRUNNER, Helen MESSIER, Reid BISSONNETTE, Fernando ECHEVERRI, Gottfried BAIER, Arun FOTEDAR, Douglas GREEN, 1994. Immunoregulatory activity of a T cell receptor alpha chain demonstrated by in vitro transcription and translation. In: Proceedings of the National Academy of Sciences of the United States of America : PNAS. 92(7), pp. 3004-3008

@article{Onda1994Immun-14327, title={Immunoregulatory activity of a T cell receptor alpha chain demonstrated by in vitro transcription and translation}, year={1994}, number={7}, volume={92}, journal={Proceedings of the National Academy of Sciences of the United States of America : PNAS}, pages={3004--3008}, author={Onda, Takebumi and Brunner, Thomas and Messier, Helen and Bissonnette, Reid and Echeverri, Fernando and Baier, Gottfried and Fotedar, Arun and Green, Douglas} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/14327"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-15T10:09:01Z</dcterms:available> <dcterms:bibliographicCitation>First publ. in: Proceedings of the National Academy of Sciences of the United States of America : PNAS ; 92 (1995), 7. - pp. 3004-3008</dcterms:bibliographicCitation> <dc:language>eng</dc:language> <dc:contributor>Messier, Helen</dc:contributor> <dc:creator>Brunner, Thomas</dc:creator> <dc:contributor>Green, Douglas</dc:contributor> <dc:creator>Echeverri, Fernando</dc:creator> <dc:contributor>Onda, Takebumi</dc:contributor> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:contributor>Baier, Gottfried</dc:contributor> <dc:contributor>Bissonnette, Reid</dc:contributor> <dc:creator>Messier, Helen</dc:creator> <dc:creator>Green, Douglas</dc:creator> <dc:contributor>Fotedar, Arun</dc:contributor> <dcterms:abstract xml:lang="eng">Previous studies from our laboratory and those of others suggested the possibility that the T-cell antigen receptor a (TCRa) chain from some T cells can be released in a soluble form and can have antigen- specific immunoregulatory activity. We have analyzed this phenomenon by in vitro transcription and translation (IVTT) of a cDNA encoding a TCRa chain (A1.1 TCRa) suspected of having such activity. We found that TCRa, but not TCR,6, protein produced in this way showed antigen-specific regulatory activity in an in vitro immune-response assay. Protein derived from truncated forms of the A1.1 TCRa cDNA had activity providing that, in addition to the variable (V) and joining (J) regions of the a chain (VJa), at least the first 25 amino acids of the a chain of the constant (C) region (Ca) were present. Addition of an irrelevant protein sequence to the VJa failed to impart activity to the molecule, suggesting that the Ca requirement is not simply for stabilization of the resulting protein. These results are discussed in the context of other recent studies on the immunoregulatory activity of soluble TCRa molecules, and the possible physiological relevance of these observations is considered.</dcterms:abstract> <dc:creator>Onda, Takebumi</dc:creator> <dc:creator>Baier, Gottfried</dc:creator> <dc:contributor>Echeverri, Fernando</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-15T10:09:01Z</dc:date> <dc:rights>deposit-license</dc:rights> <dcterms:issued>1994</dcterms:issued> <dcterms:title>Immunoregulatory activity of a T cell receptor alpha chain demonstrated by in vitro transcription and translation</dcterms:title> <dc:creator>Fotedar, Arun</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14327"/> <dc:creator>Bissonnette, Reid</dc:creator> </rdf:Description> </rdf:RDF>

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