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HIV-induced apoptosis of activated primary CD4+ T lymphocytes is not mediated by Fas-Fas ligand

HIV-induced apoptosis of activated primary CD4+ T lymphocytes is not mediated by Fas-Fas ligand

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NORAZ, Nelly, Joël GOZLAN, Jacques CORBEIL, Thomas BRUNNER, Stephen A. SPECTOR, 1997. HIV-induced apoptosis of activated primary CD4+ T lymphocytes is not mediated by Fas-Fas ligand. In: AIDS. 11(14), pp. 1671-1680. ISSN 0269-9370

@article{Noraz1997HIV-i-14311, title={HIV-induced apoptosis of activated primary CD4+ T lymphocytes is not mediated by Fas-Fas ligand}, year={1997}, doi={10.1097/00002030-199714000-00003}, number={14}, volume={11}, issn={0269-9370}, journal={AIDS}, pages={1671--1680}, author={Noraz, Nelly and Gozlan, Joël and Corbeil, Jacques and Brunner, Thomas and Spector, Stephen A.} }

Corbeil, Jacques Noraz, Nelly Brunner, Thomas 1997 deposit-license Publ. in: AIDS ; 11 (1997), 14. - S. 1671-1680 Gozlan, Joël Noraz, Nelly Brunner, Thomas eng 2011-10-24T14:35:49Z HIV-induced apoptosis of activated primary CD4+ T lymphocytes is not mediated by Fas-Fas ligand Corbeil, Jacques Spector, Stephen A. Objective:<br />To investigate the role of the Fas–Fas ligand (FasL) interaction in HIV-1-<br />induced apoptosis of primary CD4+ T lymphocytes.<br />Design:<br />Activated CD4+ T lymphocytes are the main target of HIV, and T-cell<br />activation leads to the expression of Fas–FasL and enhances HIV-mediated<br />apoptosis. Phytohemagglutinin-activated primary CD4+ T cells were infected with<br />HIV; the process of cell death was examined, and whether the dying and dead cells<br />were the productively infected cells. The modulation of Fas and FasL expression and<br />its role in HIV-induced cell death was also investigated.<br />Methods:<br />The number of viable and dead cells was determined by trypan blue<br />exclusion. Apoptosis was quantified using an enzyme-linked immunosorbent assay<br />measuring the release of cytoplasmic histone-associated DNA fragments. The<br />percentage of HIV-infected cells was determined by FACS analysis, and viral<br />production was assessed by a p24 core antigen assay. The following three markers,<br />HIV-gp-120, annexin-V and 7-AAD, were used to monitor the apoptotic process in<br />HIV-negative and positive cells. Fas and FasL expression was analyzed at the RNA<br />level by reverse transcription polymerase chain reaction and at the protein level by<br />flow cytometry. The contribution of Fas–FasL interactions to apoptosis was<br />examined by blocking experiments using the antagonist ZB4 anti-Fas antibody.<br />Results:<br />HIV-induced apoptosis in activated purified CD4+ T lymphocytes required<br />infectious virus and was dose-dependent. Apoptosis in HIV-infected cultures was<br />mostly confined to productively infected cells. The expression of Fas and FasL was<br />not significantly modulated by infection and blocking Fas–FasL interactions did not<br />reduce the extent of apoptosis.<br />Conclusions:<br />HIV-induced apoptosis of activated CD4+ T cells in vitro is confined to<br />productively infected cells and is not mediated by a Fas–FasL interaction. Spector, Stephen A. Gozlan, Joël 2011-10-24T14:35:49Z

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