Accumulation and activation-induced release of preformed Fas (CD95) ligand during the pathogenesis of experimental graft-versus-host disease

Thumbnail Image
Date
2001
Authors
Wasem, Christoph
Frutschi, Corina
Arnold, Diana
Vallan, Claudio
Lin, Tesu
Green, Douglas R.
Mueller, Christoph
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
ArXiv-ID
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published in
Journal of Immunology ; 167 (2001), 5. - pp. 2936-2941. - ISSN 0022-1767. - eISSN 1550-6606
Abstract
Fas (CD95/APO-1) ligand (FasL)-mediated cytotoxicity has been implicated in tissue destruction in a variety of diseases, including acute graft-vs-host disease (GVHD). In this study, we have analyzed FasL expression and regulation during the course of experimental murine acute GVHD. Although activation-induced FasL-mediated cytotoxicity in control T cells was sensitive to the immunosuppressant cyclosporin A, we observed that functional FasL expression of GVHD T cells became increasingly cyclosporin A unresponsive. This was found to be the result of a massive in vivo accumulation and intracellular storage of FasL protein and its release in a transcription- and protein synthesis-independent manner. Immunohistochemistry analysis of FasL expression in situ revealed accumulation of FasL-expressing cells in the spleen, the liver, and small intestine, with a typical cytoplasmic and granular expression pattern. Thus, we conclude that the release of preformed FasL by infiltrating donor T cells may contribute to recipient tissue damage during the pathogenesis of acute GVHD.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690WASEM, Christoph, Corina FRUTSCHI, Diana ARNOLD, Claudio VALLAN, Tesu LIN, Douglas R. GREEN, Christoph MUELLER, Thomas BRUNNER, 2001. Accumulation and activation-induced release of preformed Fas (CD95) ligand during the pathogenesis of experimental graft-versus-host disease. In: Journal of Immunology. 167(5), pp. 2936-2941. ISSN 0022-1767. eISSN 1550-6606. Available under: doi: 10.4049/​jimmunol.167.5.2936
BibTex
@article{Wasem2001Accum-14295,
  year={2001},
  doi={10.4049/​jimmunol.167.5.2936},
  title={Accumulation and activation-induced release of preformed Fas (CD95) ligand during the pathogenesis of experimental graft-versus-host disease},
  number={5},
  volume={167},
  issn={0022-1767},
  journal={Journal of Immunology},
  pages={2936--2941},
  author={Wasem, Christoph and Frutschi, Corina and Arnold, Diana and Vallan, Claudio and Lin, Tesu and Green, Douglas R. and Mueller, Christoph and Brunner, Thomas}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/14295">
    <dc:contributor>Lin, Tesu</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14295"/>
    <dc:creator>Arnold, Diana</dc:creator>
    <dc:creator>Vallan, Claudio</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Wasem, Christoph</dc:creator>
    <dc:contributor>Arnold, Diana</dc:contributor>
    <dc:creator>Frutschi, Corina</dc:creator>
    <dc:contributor>Green, Douglas R.</dc:contributor>
    <dc:contributor>Frutschi, Corina</dc:contributor>
    <dc:creator>Mueller, Christoph</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14295/2/2001_Wasem_Accumulation.pdf"/>
    <dcterms:abstract xml:lang="eng">Fas (CD95/APO-1) ligand (FasL)-mediated cytotoxicity has been implicated in tissue destruction in a variety of diseases, including acute graft-vs-host disease (GVHD). In this study, we have analyzed FasL expression and regulation during the course of experimental murine acute GVHD. Although activation-induced FasL-mediated cytotoxicity in control T cells was sensitive to the immunosuppressant cyclosporin A, we observed that functional FasL expression of GVHD T cells became increasingly cyclosporin A unresponsive. This was found to be the result of a massive in vivo accumulation and intracellular storage of FasL protein and its release in a transcription- and protein synthesis-independent manner. Immunohistochemistry analysis of FasL expression in situ revealed accumulation of FasL-expressing cells in the spleen, the liver, and small intestine, with a typical cytoplasmic and granular expression pattern. Thus, we conclude that the release of preformed FasL by infiltrating donor T cells may contribute to recipient tissue damage during the pathogenesis of acute GVHD.</dcterms:abstract>
    <dc:creator>Lin, Tesu</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14295/2/2001_Wasem_Accumulation.pdf"/>
    <dc:contributor>Mueller, Christoph</dc:contributor>
    <dc:creator>Brunner, Thomas</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-21T12:42:22Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-21T12:42:22Z</dc:date>
    <dcterms:title>Accumulation and activation-induced release of preformed Fas (CD95) ligand during the pathogenesis of experimental graft-versus-host disease</dcterms:title>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Vallan, Claudio</dc:contributor>
    <dc:creator>Green, Douglas R.</dc:creator>
    <dc:contributor>Wasem, Christoph</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: Journal of Immunology ; 167 (2001), 5. - S. 2936-2941</dcterms:bibliographicCitation>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Brunner, Thomas</dc:contributor>
    <dcterms:issued>2001</dcterms:issued>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No
Refereed