Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis

Cite This

Files in this item

Checksum: MD5:3e1f069030480d535dbb3148bbb48b6b

YOUSEFI, Shida, Remo PEROZZO, Inès SCHMID, Andrew ZIEMIECKI, Thomas SCHAFFNER, Leonardo SCAPOZZA, Thomas BRUNNER, Hans-Uwe SIMON, 2006. Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis. In: Nature Cell Biology. 8(10), pp. 1124-1132. ISSN 1465-7392. Available under: doi: 10.1038/ncb1482

@article{Yousefi2006-10Calpa-14275, title={Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis}, year={2006}, doi={10.1038/ncb1482}, number={10}, volume={8}, issn={1465-7392}, journal={Nature Cell Biology}, pages={1124--1132}, author={Yousefi, Shida and Perozzo, Remo and Schmid, Inès and Ziemiecki, Andrew and Schaffner, Thomas and Scapozza, Leonardo and Brunner, Thomas and Simon, Hans-Uwe} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:creator>Brunner, Thomas</dc:creator> <dcterms:rights rdf:resource=""/> <dc:contributor>Yousefi, Shida</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Nature Cell Biology ; 8 (2006), 10. - pp. 1124-1132</dcterms:bibliographicCitation> <dc:creator>Perozzo, Remo</dc:creator> <dc:language>eng</dc:language> <dc:creator>Schaffner, Thomas</dc:creator> <dc:creator>Simon, Hans-Uwe</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Schmid, Inès</dc:contributor> <dc:contributor>Perozzo, Remo</dc:contributor> <dcterms:available rdf:datatype="">2011-10-20T07:19:10Z</dcterms:available> <dcterms:issued>2006-10</dcterms:issued> <dc:contributor>Scapozza, Leonardo</dc:contributor> <dc:contributor>Simon, Hans-Uwe</dc:contributor> <bibo:uri rdf:resource=""/> <dspace:hasBitstream rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> <dcterms:hasPart rdf:resource=""/> <dspace:isPartOfCollection rdf:resource=""/> <dc:creator>Schmid, Inès</dc:creator> <dcterms:abstract xml:lang="eng">Autophagy-related gene (Atg) 5 is a gene product required for the formation of autophagosomes. Here, we report that Atg5, in addition to the promotion of autophagy, enhances susceptibility towards apoptotic stimuli. Enforced expression of Atg5-sensitized tumour cells to anticancer drug treatment both in vitro and in vivo. In contrast, silencing the Atg5 gene with short interfering RNA (siRNA) resulted in partial resistance to chemotherapy. Apoptosis was associated with calpain-mediated Atg5 cleavage, resulting in an amino-terminal cleavage product with a relative molecular mass of 24,000 (Mr 24K). Atg5 cleavage was observed independent of the cell type and the apoptotic stimulus, suggesting that calpain activation and Atg5 cleavage are general phenomena in apoptotic cells. Truncated Atg5 translocated from the cytosol to mitochondria, associated with the anti-apoptotic molecule Bcl-xL and triggered cytochrome c release and caspase activation. Taken together, calpain-mediated Atg5 cleavage provokes apoptotic cell death, therefore, represents a molecular link between autophagy and apoptosis — a finding with potential importance for clinical anticancer therapies.</dcterms:abstract> <dc:creator>Ziemiecki, Andrew</dc:creator> <dc:contributor>Schaffner, Thomas</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Ziemiecki, Andrew</dc:contributor> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:creator>Scapozza, Leonardo</dc:creator> <dc:date rdf:datatype="">2011-10-20T07:19:10Z</dc:date> <dc:creator>Yousefi, Shida</dc:creator> <dc:rights>terms-of-use</dc:rights> <dcterms:title>Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis</dcterms:title> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

2006_Yousefi-NCB.pdf 906

This item appears in the following Collection(s)

Search KOPS


My Account