KOPS - Das Institutionelle Repositorium der Universität Konstanz

Differential regulation of glucocorticoid synthesis in murine intestinal epithelial versus adrenocortical cell lines

Differential regulation of glucocorticoid synthesis in murine intestinal epithelial versus adrenocortical cell lines

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:1a9dd8cbde48e12ed50fa5a5cc0a8fa3

MUELLER, Matthias, Atanas ATANASOV, Igor CIMA, Nadia CORAZZA, Kristina SCHOONJANS, Thomas BRUNNER, 2007. Differential regulation of glucocorticoid synthesis in murine intestinal epithelial versus adrenocortical cell lines. In: Endocrinology. 148(3), pp. 1445-1453. ISSN 0013-7227. Available under: doi: 10.1210/en.2006-0591

@article{Mueller2007-03Diffe-14274, title={Differential regulation of glucocorticoid synthesis in murine intestinal epithelial versus adrenocortical cell lines}, year={2007}, doi={10.1210/en.2006-0591}, number={3}, volume={148}, issn={0013-7227}, journal={Endocrinology}, pages={1445--1453}, author={Mueller, Matthias and Atanasov, Atanas and Cima, Igor and Corazza, Nadia and Schoonjans, Kristina and Brunner, Thomas} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/14274"> <dc:creator>Mueller, Matthias</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:language>eng</dc:language> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14274/2/2007_Mueller_Diff%20reg%20glucocort.pdf"/> <dc:rights>deposit-license</dc:rights> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-19T15:03:17Z</dc:date> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:contributor>Cima, Igor</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14274/2/2007_Mueller_Diff%20reg%20glucocort.pdf"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-10-19T15:03:17Z</dcterms:available> <dc:creator>Corazza, Nadia</dc:creator> <dc:creator>Cima, Igor</dc:creator> <dcterms:issued>2007-03</dcterms:issued> <dc:contributor>Atanasov, Atanas</dc:contributor> <dc:creator>Schoonjans, Kristina</dc:creator> <dcterms:bibliographicCitation>First publ. in: Endocrinology ; 148 (2007), 3. - pp. 1445-1453</dcterms:bibliographicCitation> <dcterms:abstract xml:lang="eng">Glucocorticoids are steroid hormones with important functions in development, immune regulation, and glucose metabolism. The adrenal glands are the predominant source of glucocorticoids; however, there is increasing evidence for extraadrenal glucocorticoid synthesis in thymus, brain, skin, and vascular endothelium. We recently identified intestinal epithelial cells as an important source of glucocorticoids, which regulate the activation of local intestinal immune cells. The molecular regulation of intestinal glucocorticoid synthesis is currently unexplored. In this study we investigated the transcriptional regulation of the steroidogenic enzymes P450 side-chain cleavage enzyme and 11β-hydroxylase, and the production of corticosterone in the murine intestinal epithelial cell line mICcl2 and compared it with that in the adrenocortical cell line Y1. Surprisingly, we observed a reciprocal stimulation pattern in these two cell lines. Elevation of intracellular cAMP induced the expression of steroidogenic enzymes in Y1 cells, whereas it inhibited steroidogenesis in mICcl2 cells. In contrast, phorbol ester induced steroidogenic enzymes in intestinal epithelial cells, which was synergistically enhanced upon transfection of cells with the nuclear receptors steroidogenic factor-1 (NR5A1) and liver receptor homolog-1 (NR5A2). Finally, we observed that basal and liver receptor homolog-1/phorbol ester-induced expression of steroidogenic enzymes in mICcl2 cells was inhibited by the antagonistic nuclear receptor small heterodimer partner. We conclude that the molecular basis of glucocorticoid synthesis in intestinal epithelial cells is distinct from that in adrenal cells, most likely representing an adaptation to the local environment and different requirements.</dcterms:abstract> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Atanasov, Atanas</dc:creator> <dc:contributor>Schoonjans, Kristina</dc:contributor> <dc:contributor>Corazza, Nadia</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14274"/> <dc:contributor>Mueller, Matthias</dc:contributor> <dcterms:title>Differential regulation of glucocorticoid synthesis in murine intestinal epithelial versus adrenocortical cell lines</dcterms:title> <dc:contributor>Brunner, Thomas</dc:contributor> <dc:creator>Brunner, Thomas</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

2007_Mueller_Diff reg glucocort.pdf 271

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto