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Investigation of electrogenic partial reactions in detergent-solubilized Na,K-ATPase

Investigation of electrogenic partial reactions in detergent-solubilized Na,K-ATPase


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HABECK, Michael, Erica CIRRI, Adriana KATZ, Steven J. KARLISH, Hans-Jürgen APELL, 2009. Investigation of electrogenic partial reactions in detergent-solubilized Na,K-ATPase. In: Biochemistry. 48(38), pp. 9147-9155. ISSN 0006-2960. eISSN 1520-4995

@article{Habeck2009-09-29Inves-14176, title={Investigation of electrogenic partial reactions in detergent-solubilized Na,K-ATPase}, year={2009}, doi={10.1021/bi901148k}, number={38}, volume={48}, issn={0006-2960}, journal={Biochemistry}, pages={9147--9155}, author={Habeck, Michael and Cirri, Erica and Katz, Adriana and Karlish, Steven J. and Apell, Hans-Jürgen} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/14176"> <dc:creator>Habeck, Michael</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:creator>Karlish, Steven J.</dc:creator> <dcterms:bibliographicCitation>First publ. in: Biochemistry ; 48 (2009), 38. - pp. 9147-9155</dcterms:bibliographicCitation> <dc:contributor>Apell, Hans-Jürgen</dc:contributor> <dcterms:title>Investigation of electrogenic partial reactions in detergent-solubilized Na,K-ATPase</dcterms:title> <dc:contributor>Cirri, Erica</dc:contributor> <dc:creator>Apell, Hans-Jürgen</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-09-22T07:38:01Z</dcterms:available> <dc:contributor>Katz, Adriana</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14176"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-09-22T07:38:01Z</dc:date> <dcterms:abstract xml:lang="eng">A method to investigate electrogenic partial reactions in the pump cycle of membrane-bound P-type ATPases with electrochromic fluorescent dyes has been extended to detergent-solubilized native and purified recombinant Na,K-ATPase. As a first step, it has been shown here that the function and ion-binding properties of the detergent-soluble and membrane-bound rabbit renal Na,K-ATPase are not significantly different. Thus, the new assay overcomes a previous limitation of the styryl dye method, in that that the protein need not be embedded in a membrane at a high density. As an example of an application of this method, transport properties of recombinant Na,K-ATPase purified from yeast cells have been studied. We have investigated and compared Na+ and K+-binding properties of purified detergent-soluble human α1/his-β1 and α2/his-β1 isoforms of the sodium pump. The only significant difference found with respect to ion binding between both isoforms is an almost three-fold lower affinity for K+ binding in the E2P state of the α2/his-β1 isoform. This technique should be readily applicable to various other P-type ATPases, or transport proteins such as carriers or ion channels that can be purified in a detergent-soluble active form.</dcterms:abstract> <dc:contributor>Karlish, Steven J.</dc:contributor> <dcterms:issued>2009-09-29</dcterms:issued> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:creator>Cirri, Erica</dc:creator> <dc:language>eng</dc:language> <dc:creator>Katz, Adriana</dc:creator> <dc:contributor>Habeck, Michael</dc:contributor> </rdf:Description> </rdf:RDF>

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