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Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity

Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity

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Prüfsumme: MD5:275d9c2800d2ad5f2191b922ae47d7ef

SCHMIDT, Andreas, Peter I. DUNCAN, Nadine R. RAUH, Guido SAUER, Andrew M. FRY, Erich A. NIGG, Thomas MAYER, 2005. Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity. In: Genes & Development. 19(4), pp. 502-513. ISSN 0890-9369

@article{Schmidt2005-02-15Xenop-14048, title={Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity}, year={2005}, doi={10.1101/gad.320705}, number={4}, volume={19}, issn={0890-9369}, journal={Genes & Development}, pages={502--513}, author={Schmidt, Andreas and Duncan, Peter I. and Rauh, Nadine R. and Sauer, Guido and Fry, Andrew M. and Nigg, Erich A. and Mayer, Thomas} }

Metaphase-to-anaphase transition is a fundamental step in cell cycle progression where duplicated sister-chromatids segregate to the future daughter cells. The anaphase-promoting complex/cyclosome (APC/C) is a highly regulated ubiquitin-ligase that triggers anaphase onset and mitotic exit by targeting securin and mitotic cyclins for destruction. It was previously shown that the Xenopus polo-like kinase Plx1 is essential to activate APC/C upon release from cytostatic factor (CSF) arrest in Xenopus egg extract. Although the mechanism by which Plx1 regulates APC/C activation remained unclear, the existence of a putative APC/C inhibitor was postulated whose activity would be neutralized by Plx1 upon CSF release. Here we identify XErp1, a novel Plx1-regulated inhibitor of APC/C activity, and we demonstrate that XErp1 is required to prevent anaphase onset in CSF-arrested Xenopus egg extract. Inactivation of XErp1 leads to premature APC/C activation. Conversely, addition of excess XErp1 to Xenopus egg extract prevents APC/C activation. Plx1 phosphorylates XErp1 in vitro at a site that targets XErp1 for degradation upon CSF release. Thus, our data lead to a model of APC/C activation in Xenopus egg extract in which Plx1 targets the APC/C inhibitor XErp1 for degradation. Mayer, Thomas Nigg, Erich A. 2012-01-11T09:36:03Z Schmidt, Andreas Sauer, Guido Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity Sauer, Guido Fry, Andrew M. Rauh, Nadine R. Rauh, Nadine R. Duncan, Peter I. Fry, Andrew M. Duncan, Peter I. Schmidt, Andreas First publ. in: Genes & development ; 19 (2005), 4. - S. 502-513 deposit-license eng 2005-02-15 2012-01-11T09:36:03Z Mayer, Thomas Nigg, Erich A.

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