Type of Publication: | Journal article |
URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-140483 |
Author: | Schmidt, Andreas; Duncan, Peter I.; Rauh, Nadine R.; Sauer, Guido; Fry, Andrew M.; Nigg, Erich A.; Mayer, Thomas U. |
Year of publication: | 2005 |
Published in: | Genes & Development ; 19 (2005), 4. - pp. 502-513. - ISSN 0890-9369 |
Pubmed ID: | 15713843 |
DOI (citable link): | https://dx.doi.org/10.1101/gad.320705 |
Summary: |
Metaphase-to-anaphase transition is a fundamental step in cell cycle progression where duplicated sister-chromatids segregate to the future daughter cells. The anaphase-promoting complex/cyclosome (APC/C) is a highly regulated ubiquitin-ligase that triggers anaphase onset and mitotic exit by targeting securin and mitotic cyclins for destruction. It was previously shown that the Xenopus polo-like kinase Plx1 is essential to activate APC/C upon release from cytostatic factor (CSF) arrest in Xenopus egg extract. Although the mechanism by which Plx1 regulates APC/C activation remained unclear, the existence of a putative APC/C inhibitor was postulated whose activity would be neutralized by Plx1 upon CSF release. Here we identify XErp1, a novel Plx1-regulated inhibitor of APC/C activity, and we demonstrate that XErp1 is required to prevent anaphase onset in CSF-arrested Xenopus egg extract. Inactivation of XErp1 leads to premature APC/C activation. Conversely, addition of excess XErp1 to Xenopus egg extract prevents APC/C activation. Plx1 phosphorylates XErp1 in vitro at a site that targets XErp1 for degradation upon CSF release. Thus, our data lead to a model of APC/C activation in Xenopus egg extract in which Plx1 targets the APC/C inhibitor XErp1 for degradation.
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Subject (DDC): | 570 Biosciences, Biology |
Controlled Keywords (GND): | Meiose Ubiquitin-Protein-Ligase |
Keywords: | APC/C, metaphase II, Meiose, Ubiquitin-Protein-Ligase, Cell cycle, anaphase-promoting complex/cyclosome, Xenopus, polo-like kinase, cytostatic factor, mitotic exit |
Link to License: | In Copyright |
SCHMIDT, Andreas, Peter I. DUNCAN, Nadine R. RAUH, Guido SAUER, Andrew M. FRY, Erich A. NIGG, Thomas U. MAYER, 2005. Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity. In: Genes & Development. 19(4), pp. 502-513. ISSN 0890-9369. Available under: doi: 10.1101/gad.320705
@article{Schmidt2005-02-15Xenop-14048, title={Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activity}, year={2005}, doi={10.1101/gad.320705}, number={4}, volume={19}, issn={0890-9369}, journal={Genes & Development}, pages={502--513}, author={Schmidt, Andreas and Duncan, Peter I. and Rauh, Nadine R. and Sauer, Guido and Fry, Andrew M. and Nigg, Erich A. and Mayer, Thomas U.} }
Mayer etal.pdf | 1152 |