Site-specific DNA labelling by Staudinger ligation

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WEISBROD, Samuel H., Anna BACCARO, Andreas MARX, WALKER, John, ed., 2011. Site-specific DNA labelling by Staudinger ligation. In: MARK, Sonny S., ed.. Bioconjugation Protocols. Totowa, NJ:Humana Press, pp. 195-207. ISBN 978-1-61779-150-5

@incollection{Weisbrod2011Site--14013, title={Site-specific DNA labelling by Staudinger ligation}, year={2011}, doi={10.1007/978-1-61779-151-2_12}, number={751}, isbn={978-1-61779-150-5}, address={Totowa, NJ}, publisher={Humana Press}, series={Methods in Molecular Biology}, booktitle={Bioconjugation Protocols}, pages={195--207}, editor={Mark, Sonny S.}, author={Weisbrod, Samuel H. and Baccaro, Anna and Marx, Andreas} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/14013"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-07-19T08:19:27Z</dcterms:available> <dc:rights>deposit-license</dc:rights> <dc:creator>Marx, Andreas</dc:creator> <dc:creator>Weisbrod, Samuel H.</dc:creator> <dc:contributor>Walker, John</dc:contributor> <dcterms:title>Site-specific DNA labelling by Staudinger ligation</dcterms:title> <dcterms:abstract xml:lang="eng">Site-specific and chemoselective labeling of DNA is still a difficult task. The Staudinger ligation is a bioorthogonal reaction between azides and phosphines that requires no catalyst to proceed, allowing for mild reaction conditions. The reaction may be extended for site-specific labeling of DNA using azidomodified triphosphates, which can be incorporated site-specifically into DNA strands by DNA polymerases in a template-dependent manner. The azido-modified DNA, in turn, can be labeled by suitable phosphines. This protocol describes (1) the synthesis of an azido-TTP analogue; (2) the enzymatic synthesis of azido-modified DNA; (3) the synthesis of suitable phosphine labels; and (4) the labeling of azido-DNA with biotin–phosphine by Staudinger ligation with approximately 70% conversion.</dcterms:abstract> <dc:contributor>Baccaro, Anna</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14013"/> <dcterms:bibliographicCitation>Publ. in: Bioconjugation Protocols / ed. by Sonny S. Mark (Methods in Molecular Biology, Vol. 751). Totowa, NJ : Humana Press, 2011, 2. Ed., pp. 195-207</dcterms:bibliographicCitation> <dc:creator>Baccaro, Anna</dc:creator> <dc:language>eng</dc:language> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-07-19T08:19:27Z</dc:date> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:contributor>Marx, Andreas</dc:contributor> <dcterms:issued>2011</dcterms:issued> <dc:contributor>Weisbrod, Samuel H.</dc:contributor> </rdf:Description> </rdf:RDF>

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