Site-specific DNA labelling by Staudinger ligation


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WEISBROD, Samuel H., Anna BACCARO, Andreas MARX, WALKER, John, ed., 2011. Site-specific DNA labelling by Staudinger ligation. In: MARK, Sonny S., ed.. Bioconjugation Protocols. Totowa, NJ:Humana Press, pp. 195-207. ISBN 978-1-61779-150-5. Available under: doi: 10.1007/978-1-61779-151-2_12

@incollection{Weisbrod2011Sites-14013, title={Site-specific DNA labelling by Staudinger ligation}, year={2011}, doi={10.1007/978-1-61779-151-2_12}, number={751}, isbn={978-1-61779-150-5}, address={Totowa, NJ}, publisher={Humana Press}, series={Methods in Molecular Biology}, booktitle={Bioconjugation Protocols}, pages={195--207}, editor={Mark, Sonny S.}, author={Weisbrod, Samuel H. and Baccaro, Anna and Marx, Andreas} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dcterms:available rdf:datatype="">2011-07-19T08:19:27Z</dcterms:available> <dc:creator>Marx, Andreas</dc:creator> <dc:creator>Weisbrod, Samuel H.</dc:creator> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Walker, John</dc:contributor> <dcterms:title>Site-specific DNA labelling by Staudinger ligation</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <bibo:uri rdf:resource=""/> <dc:contributor>Baccaro, Anna</dc:contributor> <dcterms:abstract xml:lang="eng">Site-specific and chemoselective labeling of DNA is still a difficult task. The Staudinger ligation is a bioorthogonal reaction between azides and phosphines that requires no catalyst to proceed, allowing for mild reaction conditions. The reaction may be extended for site-specific labeling of DNA using azidomodified triphosphates, which can be incorporated site-specifically into DNA strands by DNA polymerases in a template-dependent manner. The azido-modified DNA, in turn, can be labeled by suitable phosphines. This protocol describes (1) the synthesis of an azido-TTP analogue; (2) the enzymatic synthesis of azido-modified DNA; (3) the synthesis of suitable phosphine labels; and (4) the labeling of azido-DNA with biotin–phosphine by Staudinger ligation with approximately 70% conversion.</dcterms:abstract> <dcterms:bibliographicCitation>Publ. in: Bioconjugation Protocols / ed. by Sonny S. Mark (Methods in Molecular Biology, Vol. 751). Totowa, NJ : Humana Press, 2011, 2. Ed., pp. 195-207</dcterms:bibliographicCitation> <dc:creator>Baccaro, Anna</dc:creator> <dcterms:rights rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> <dc:language>eng</dc:language> <dc:date rdf:datatype="">2011-07-19T08:19:27Z</dc:date> <dc:contributor>Marx, Andreas</dc:contributor> <dcterms:issued>2011</dcterms:issued> <dc:contributor>Weisbrod, Samuel H.</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dspace:isPartOfCollection rdf:resource=""/> </rdf:Description> </rdf:RDF>

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