Site-specific labeling of proteins with NMR-active unnatural amino acids


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JONES, David H., Susan E. CELLITTI, Xueshi HAO, Qiong ZHANG, Michael JAHNZ, Daniel SUMMERER, Peter G. SCHULTZ, Tetsuo UNO, Bernhard H. GEIERSTANGER, 2010. Site-specific labeling of proteins with NMR-active unnatural amino acids. In: Journal of Biomolecular NMR. 46(1), pp. 89-100. ISSN 0925-2738. eISSN 1573-5001. Available under: doi: 10.1007/s10858-009-9365-4

@article{Jones2010-01Sites-13747, title={Site-specific labeling of proteins with NMR-active unnatural amino acids}, year={2010}, doi={10.1007/s10858-009-9365-4}, number={1}, volume={46}, issn={0925-2738}, journal={Journal of Biomolecular NMR}, pages={89--100}, author={Jones, David H. and Cellitti, Susan E. and Hao, Xueshi and Zhang, Qiong and Jahnz, Michael and Summerer, Daniel and Schultz, Peter G. and Uno, Tetsuo and Geierstanger, Bernhard H.} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:contributor>Schultz, Peter G.</dc:contributor> <dc:creator>Uno, Tetsuo</dc:creator> <dcterms:isPartOf rdf:resource=""/> <dcterms:available rdf:datatype="">2011-11-11T09:04:54Z</dcterms:available> <dcterms:abstract xml:lang="eng">A large number of amino acids other than the canonical amino acids can now be easily incorporated in vivo into proteins at genetically encoded positions. The technology requires an orthogonal tRNA/aminoacyl-tRNA synthetase pair specific for the unnatural amino acid that is added to the media while a TAG amber or frame shift codon specifies the incorporation site in the protein to be studied. These unnatural amino acids can be isotopically labeled and provide unique opportunities for site-specific labeling of proteins for NMR studies. In this perspective, we discuss these opportunities including new photocaged unnatural amino acids, outline usage of metal chelating and spin-labeled unnatural amino acids and expand the approach to in-cell NMR experiments.</dcterms:abstract> <dc:date rdf:datatype="">2011-11-11T09:04:54Z</dc:date> <dc:contributor>Summerer, Daniel</dc:contributor> <dc:creator>Zhang, Qiong</dc:creator> <bibo:uri rdf:resource=""/> <dc:creator>Cellitti, Susan E.</dc:creator> <dc:rights>terms-of-use</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Geierstanger, Bernhard H.</dc:creator> <dc:contributor>Hao, Xueshi</dc:contributor> <dc:contributor>Jones, David H.</dc:contributor> <dc:contributor>Zhang, Qiong</dc:contributor> <dc:creator>Schultz, Peter G.</dc:creator> <dc:contributor>Cellitti, Susan E.</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Uno, Tetsuo</dc:contributor> <dcterms:issued>2010-01</dcterms:issued> <dspace:hasBitstream rdf:resource=""/> <dc:creator>Summerer, Daniel</dc:creator> <dcterms:title>Site-specific labeling of proteins with NMR-active unnatural amino acids</dcterms:title> <dcterms:hasPart rdf:resource=""/> <dc:contributor>Jahnz, Michael</dc:contributor> <dc:creator>Jones, David H.</dc:creator> <dc:creator>Hao, Xueshi</dc:creator> <dcterms:bibliographicCitation>First publ. in: Journal of Biomolecular NMR ; 46 (2010), 1. - S. 89-100</dcterms:bibliographicCitation> <dc:contributor>Geierstanger, Bernhard H.</dc:contributor> <dc:language>eng</dc:language> <dcterms:rights rdf:resource=""/> <dc:creator>Jahnz, Michael</dc:creator> <dspace:isPartOfCollection rdf:resource=""/> </rdf:Description> </rdf:RDF>

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