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A Direct and Functional Interaction Between Go and Rab5 During G Protein-Coupled Receptor Signaling

A Direct and Functional Interaction Between Go and Rab5 During G Protein-Coupled Receptor Signaling

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Prüfsumme: MD5:6617f148d135c8f0706405dadf160764
Prüfsumme: MD5:72c43a5a51a7b4f2a59ea682c8bd5bf1

PURVANOV, Vladimir, Alexey KOVAL, Vladimir KATANAEV, 2010. A Direct and Functional Interaction Between Go and Rab5 During G Protein-Coupled Receptor Signaling. In: Science Signaling. 3(136), pp. ra65-ra65. eISSN 1937-9145

@article{Purvanov2010Direc-13576, title={A Direct and Functional Interaction Between Go and Rab5 During G Protein-Coupled Receptor Signaling}, year={2010}, doi={10.1126/scisignal.2000877}, number={136}, volume={3}, journal={Science Signaling}, pages={ra65--ra65}, author={Purvanov, Vladimir and Koval, Alexey and Katanaev, Vladimir} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/13576"> <dc:contributor>Purvanov, Vladimir</dc:contributor> <dcterms:title>A Direct and Functional Interaction Between Go and Rab5 During G Protein-Coupled Receptor Signaling</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-31T22:25:05Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-06-09T08:22:43Z</dc:date> <dc:contributor>Katanaev, Vladimir</dc:contributor> <dc:creator>Koval, Alexey</dc:creator> <dc:creator>Purvanov, Vladimir</dc:creator> <dc:contributor>Koval, Alexey</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/13576"/> <dcterms:bibliographicCitation>First publ. in: Science Signaling 3 (2010), 136, p. ra65, DOI: 10.1126/scisignal.2000877</dcterms:bibliographicCitation> <dc:creator>Katanaev, Vladimir</dc:creator> <dc:language>eng</dc:language> <dcterms:abstract xml:lang="eng">Rab5 is a small guanosine triphosphatase (GTPase) that regulates the early stages of endocytosis and is conserved in eukaryotes. Rab5 regulates the internalization of receptors and other membrane-associated signaling proteins. The function of Rab5 in these processes is considered relatively passive, so that the endocytic capacity of Rab5 is used during, for example, β-arrestin–dependent internalization of G protein (heterotrimeric guanine nucleotide–binding protein)–coupled receptors (GPCRs). Direct recruitment or activation of Rab5 by the components of these signaling pathways has not been reported. Here, we demonstrate an interaction of Drosophila Rab5 and an immediate transducer of GPCR signaling, the G protein Go, in vitro and in vivo. Rab5 and Go bound to each other as purified proteins, as well as in fly extracts. In cellular assays, Go led to Rab5 activation and endosome fusion. We further showed that the Go-Rab5 interaction functioned in Drosophila planar cell polarity and Wingless signal transduction, pathways initiated by GPCRs of the Frizzled (Fz) family. Additionally, the recycling Rab GTPases Rab4 and Rab11 functioned in Fz- and Go-mediated signaling to favor planar cell polarity over canonical Wingless signaling. The interplay between heterotrimeric G proteins and Rab GTPases controlled receptor internalization, revealing a previously uncharacterized regulatory mechanism in GPCR signaling.</dcterms:abstract> <dc:rights>deposit-license</dc:rights> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dcterms:issued>2010</dcterms:issued> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

ra65.pdf 195
suppmaterialpurvanovetal.pdf 35

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