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Internalization and Coreceptor Expression Are Critical for TLR2-Mediated Recognition of Lipoteichoic Acid in Human Peripheral Blood

Internalization and Coreceptor Expression Are Critical for TLR2-Mediated Recognition of Lipoteichoic Acid in Human Peripheral Blood

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BUNK, Sebastian, Stefanie SIGEL, Daniela METZDORF, Omar SHARIF, Kathy TRIANTAFILOU, Martha TRIANTAFILOU, Thomas HARTUNG, Sylvia KNAPP, Sonja VON AULOCK, 2010. Internalization and Coreceptor Expression Are Critical for TLR2-Mediated Recognition of Lipoteichoic Acid in Human Peripheral Blood. In: The Journal of Immunology. 185(6), pp. 3708-3717. ISSN 0022-1767. eISSN 1550-6606

@article{Bunk2010Inter-13534, title={Internalization and Coreceptor Expression Are Critical for TLR2-Mediated Recognition of Lipoteichoic Acid in Human Peripheral Blood}, year={2010}, doi={10.4049/jimmunol.0901660}, number={6}, volume={185}, issn={0022-1767}, journal={The Journal of Immunology}, pages={3708--3717}, author={Bunk, Sebastian and Sigel, Stefanie and Metzdorf, Daniela and Sharif, Omar and Triantafilou, Kathy and Triantafilou, Martha and Hartung, Thomas and Knapp, Sylvia and von Aulock, Sonja} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/13534"> <dcterms:abstract xml:lang="eng">Lipoteichoic acid (LTA), a ubiquitous cell wall component of Gram-positive bacteria, represents a potent immunostimulatory molecule. Because LTA of a mutant Staphylococcus aureus strain lacking lipoproteins (Δlgt-LTA) has been described to be immunobiologically inactive despite a lack of ascertained structural differences to wild-type LTA (wt-LTA), we investigated the functional requirements for the recognition of Δlgt-LTA by human peripheral blood cells. In this study, we demonstrate that Δlgt-LTA–induced immune activation critically depends on the immobilization of LTA and the presence of human serum components, which, to a lesser degree, was also observed for wt-LTA. Under experimental conditions allowing LTA-mediated stimulation, we found no differences between the immunostimulatory capacity of Δlgt-LTA and wt-LTA in human blood cells, arguing for a limited contribution of possible lipoprotein contaminants to wt-LTA–mediated immune activation. In contrast to human blood cells, TLR2-transfected human embryonic kidney 293 cells could be activated only by wt-LTA, whereas activation of these cells by Δlgt-LTA required the additional expression of TLR6 and CD14, suggesting that activation of human embryonic kidney 293 cells expressing solely TLR2 is probably mediated by residual lipoproteins in wt-LTA. Notably, in human peripheral blood, LTA-specific IgG Abs are essential for Δlgt-LTA–mediated immune activation and appear to induce the phagocytic uptake of Δlgt-LTA via engagement of FcγRII. In this study, we have elucidated a novel mechanism of LTA-induced cytokine induction in human peripheral blood cells that involves uptake of LTA and subsequent intracellular recognition driven by TLR2, TLR6, and CD14.</dcterms:abstract> <dcterms:bibliographicCitation>First publ. in: The Journal of Immunology 185 (2010), 6, pp. 3708-3717</dcterms:bibliographicCitation> <dc:contributor>Sigel, Stefanie</dc:contributor> <dcterms:title>Internalization and Coreceptor Expression Are Critical for TLR2-Mediated Recognition of Lipoteichoic Acid in Human Peripheral Blood</dcterms:title> <dc:creator>Sharif, Omar</dc:creator> <dc:contributor>Metzdorf, Daniela</dc:contributor> <dc:creator>Sigel, Stefanie</dc:creator> <dc:creator>Hartung, Thomas</dc:creator> <dc:creator>Metzdorf, Daniela</dc:creator> <dc:creator>Triantafilou, Martha</dc:creator> <dc:creator>Knapp, Sylvia</dc:creator> <dc:contributor>Triantafilou, Martha</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:contributor>Sharif, Omar</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/13534"/> <dc:contributor>Triantafilou, Kathy</dc:contributor> <dcterms:issued>2010</dcterms:issued> <dc:contributor>Bunk, Sebastian</dc:contributor> <dc:creator>Bunk, Sebastian</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-31T22:25:05Z</dcterms:available> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-05-30T07:42:25Z</dc:date> <dc:creator>Triantafilou, Kathy</dc:creator> <dc:contributor>Knapp, Sylvia</dc:contributor> <dc:creator>von Aulock, Sonja</dc:creator> <dc:contributor>von Aulock, Sonja</dc:contributor> <dc:contributor>Hartung, Thomas</dc:contributor> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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