KOPS - Das Institutionelle Repositorium der Universität Konstanz

Binding regions of outer membrane protein A in complexes with the periplasmic chaperone Skp : a site-directed fluorescence study

Binding regions of outer membrane protein A in complexes with the periplasmic chaperone Skp : a site-directed fluorescence study

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

QU, Jian, Susanne BEHRENS-KNEIP, Otto HOLST, Jörg H. KLEINSCHMIDT, 2009. Binding regions of outer membrane protein A in complexes with the periplasmic chaperone Skp : a site-directed fluorescence study. In: Biochemistry. 48(22), pp. 4926-4936. ISSN 0006-2960. eISSN 1520-4995

@article{Qu2009Bindi-1275, title={Binding regions of outer membrane protein A in complexes with the periplasmic chaperone Skp : a site-directed fluorescence study}, year={2009}, doi={10.1021/bi9004039}, number={22}, volume={48}, issn={0006-2960}, journal={Biochemistry}, pages={4926--4936}, author={Qu, Jian and Behrens-Kneip, Susanne and Holst, Otto and Kleinschmidt, Jörg H.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1275"> <dc:creator>Qu, Jian</dc:creator> <dc:contributor>Holst, Otto</dc:contributor> <dcterms:abstract xml:lang="eng">Periplasmic Skp facilitates folding and membrane insertion of many outer membrane proteins (OMPs) into the outer membrane of Gram-negative bacteria. We have examined the binding sites of outer membrane protein A (OmpA) from Escherichia coli in its complexes with the membrane protein chaperone Skp and with Skp and lipopolysaccharide (LPS) by site-directed fluorescence spectroscopy. Single-Trp OmpA mutants, Wn-OmpA, with tryptophan at position n in the polypeptide chain were isolated in unfolded form in 8 M urea. In five βxWn-OmpA mutants, the tryptophan was located in β-strand x, in four lyWn-OmpA mutants, in outer loop y, and in three tzWn-OmpA mutants in turn z of the β-barrel transmembrane domain (TMD) of OmpA. PDW286-OmpA contained tryptophan in the periplasmic domain (PD). After dilution of the denaturant urea in aqueous solution, spectra indicated a more hydrophobic environment of the tryptophans in βxWn mutants in comparison to lyWn-OmpA and tzWn-OmpA, indicating that the loops and turns form the surface of hydrophobically collapsed OmpA, while the strand regions are less exposed to water. Addition of Skp increased the fluorescence of all OmpA mutants except PDW286-OmpA, demonstrating binding of Skp to the entire β-barrel domain, but not to the PD of OmpA. Skp bound the TMD of OmpA asymmetrically, displaying much stronger interactions with strands β1 to β3 in the N- than with strands β5 to β7 in the C-terminus. This asymmetry was not observed for the outer loops and the periplasmic turns of the TMD of OmpA. The fluorescence results demonstrated that all turns and loops l1, l2, and l4 were as strongly bound to Skp as the N-terminal β-strands. Addition of five negatively charged LPS per one preformed Skp-Wn-OmpA complex released the C-terminal loops l2, l3, and l4 of the TMD of OmpA from the complex, while its periplasmic turn regions remained bound to Skp. Our results demonstrate that interactions of Skp-OmpA complexes with LPS change the conformation of OmpA in the Skp complex for facilitated insertion and folding into membranes.</dcterms:abstract> <dcterms:title>Binding regions of outer membrane protein A in complexes with the periplasmic chaperone Skp : a site-directed fluorescence study</dcterms:title> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:creator>Kleinschmidt, Jörg H.</dc:creator> <dc:contributor>Kleinschmidt, Jörg H.</dc:contributor> <dcterms:bibliographicCitation>First publ. in: Biochemistry 48 (2009), 22, pp. 4926 4936</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:34Z</dcterms:available> <dc:contributor>Behrens-Kneip, Susanne</dc:contributor> <dcterms:issued>2009</dcterms:issued> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1275"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:34Z</dc:date> <dc:creator>Holst, Otto</dc:creator> <dc:contributor>Qu, Jian</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:creator>Behrens-Kneip, Susanne</dc:creator> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto