Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells

No Thumbnail Available
Files
There are no files associated with this item.
Date
2009
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
DOI (citable link)
ArXiv-ID
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published in
BioTechniques ; 46 (2009), 1. - pp. 21-28. - ISSN 0736-6205. - eISSN 1940-9818
Abstract
Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690MATENTZOGLU, Konstantin, Martin SCHEFFNER, 2009. Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells. In: BioTechniques. 46(1), pp. 21-28. ISSN 0736-6205. eISSN 1940-9818. Available under: doi: 10.2144/000113023
BibTex
@article{Matentzoglu2009Ubiqu-1271,
  year={2009},
  doi={10.2144/000113023},
  title={Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells},
  number={1},
  volume={46},
  issn={0736-6205},
  journal={BioTechniques},
  pages={21--28},
  author={Matentzoglu, Konstantin and Scheffner, Martin}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/1271">
    <dc:contributor>Scheffner, Martin</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dcterms:available>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dc:date>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1271"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:title>Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells</dcterms:title>
    <dc:language>eng</dc:language>
    <dc:creator>Scheffner, Martin</dc:creator>
    <dcterms:abstract xml:lang="eng">Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest.</dcterms:abstract>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2009</dcterms:issued>
    <dc:contributor>Matentzoglu, Konstantin</dc:contributor>
    <dc:creator>Matentzoglu, Konstantin</dc:creator>
    <dcterms:bibliographicCitation>Publ. in: BioTechniques 46 (2009), 1, pp. 21-28</dcterms:bibliographicCitation>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes
Refereed