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Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells

Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells

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MATENTZOGLU, Konstantin, Martin SCHEFFNER, 2009. Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells. In: BioTechniques. 46(1), pp. 21-28. ISSN 0736-6205. eISSN 1940-9818

@article{Matentzoglu2009Ubiqu-1271, title={Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells}, year={2009}, number={1}, volume={46}, issn={0736-6205}, journal={BioTechniques}, pages={21--28}, author={Matentzoglu, Konstantin and Scheffner, Martin} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1271"> <dcterms:title>Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells</dcterms:title> <dc:contributor>Scheffner, Martin</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dcterms:available> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1271"/> <dc:language>eng</dc:language> <dc:creator>Matentzoglu, Konstantin</dc:creator> <dcterms:issued>2009</dcterms:issued> <dcterms:abstract xml:lang="eng">Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest.</dcterms:abstract> <dc:rights>deposit-license</dc:rights> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dcterms:bibliographicCitation>Publ. in: BioTechniques 46 (2009), 1, pp. 21-28</dcterms:bibliographicCitation> <dc:creator>Scheffner, Martin</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:33Z</dc:date> <dc:contributor>Matentzoglu, Konstantin</dc:contributor> </rdf:Description> </rdf:RDF>

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