Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells
| Type of Publication: | Journal article |
| Author: | Matentzoglu, Konstantin; Scheffner, Martin |
| Year of publication: | 2009 |
| Published in: | BioTechniques ; 46 (2009), 1. - pp. 21-28. - ISSN 0736-6205. - eISSN 1940-9818 |
| DOI (citable link): | https://dx.doi.org/10.2144/000113023 |
| Summary: |
Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest.
|
| Subject (DDC): | 570 Biosciences, Biology |
| Controlled Keywords (GND): | Ubiquitin |
| Bibliography of Konstanz: | Yes |
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MATENTZOGLU, Konstantin, Martin SCHEFFNER, 2009. Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells. In: BioTechniques. 46(1), pp. 21-28. ISSN 0736-6205. eISSN 1940-9818. Available under: doi: 10.2144/000113023
@article{Matentzoglu2009Ubiqu-1271, title={Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells}, year={2009}, doi={10.2144/000113023}, number={1}, volume={46}, issn={0736-6205}, journal={BioTechniques}, pages={21--28}, author={Matentzoglu, Konstantin and Scheffner, Martin} }
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