@article{Matentzoglu2009Ubiqu-1271,
  year={2009},
  doi={10.2144/000113023},
  title={Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells},
  number={1},
  volume={46},
  issn={0736-6205},
  journal={BioTechniques},
  pages={21--28},
  author={Matentzoglu, Konstantin and Scheffner, Martin}
}

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    <dc:contributor>Scheffner, Martin</dc:contributor>
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    <dcterms:title>Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells</dcterms:title>
    <dc:language>eng</dc:language>
    <dc:creator>Scheffner, Martin</dc:creator>
    <dcterms:abstract xml:lang="eng">Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest.</dcterms:abstract>
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    <dcterms:issued>2009</dcterms:issued>
    <dc:contributor>Matentzoglu, Konstantin</dc:contributor>
    <dc:creator>Matentzoglu, Konstantin</dc:creator>
    <dcterms:bibliographicCitation>Publ. in: BioTechniques 46 (2009), 1, pp. 21-28</dcterms:bibliographicCitation>
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