Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells
Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells
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2009
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BioTechniques ; 46 (2009), 1. - pp. 21-28. - ISSN 0736-6205. - eISSN 1940-9818
Abstract
Clonal cell lines stably expressing a protein of interest are frequently used in the characterization of the potential physiological functions of mammalian proteins, but the generation of such cell lines is laborious and time-consuming. Here, we made use of the fact that fusion proteins consisting of ubiquitin linked to the N terminus of a protein of interest are efficiently processed by ubiquitin-specific proteases into their respective free proteins within eukaryotic cells, and designed an expression system that allows easy and highly efficient selection of mammalian cells ectopically expressing a protein of interest.
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MATENTZOGLU, Konstantin, Martin SCHEFFNER, 2009. Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells. In: BioTechniques. 46(1), pp. 21-28. ISSN 0736-6205. eISSN 1940-9818. Available under: doi: 10.2144/000113023BibTex
@article{Matentzoglu2009Ubiqu-1271, year={2009}, doi={10.2144/000113023}, title={Ubiquitin-fusion protein system : a powerful tool for ectopic protein expression in mammalian cells}, number={1}, volume={46}, issn={0736-6205}, journal={BioTechniques}, pages={21--28}, author={Matentzoglu, Konstantin and Scheffner, Martin} }
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