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Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains

Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains

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Prüfsumme: MD5:8a84590be17ec9e9a8b0a27bfda4077d

WEIDMANN, Manfred, Ousmane FAYE, Oumar FAYE, Ramon KRANASTER, Andreas MARX, Marcio R. T. NUNES, Pedro F. C. VASCONCELOS, Frank T. HUFERT, Amadou A. SALL, 2010. Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains. In: Journal of Clinical Virology. 48(3), pp. 187-192. ISSN 1386-6532. eISSN 1873-5967

@article{Weidmann2010Impro-12601, title={Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains}, year={2010}, doi={10.1016/j.jcv.2010.04.013}, number={3}, volume={48}, issn={1386-6532}, journal={Journal of Clinical Virology}, pages={187--192}, author={Weidmann, Manfred and Faye, Ousmane and Faye, Oumar and Kranaster, Ramon and Marx, Andreas and Nunes, Marcio R. T. and Vasconcelos, Pedro F. C. and Hufert, Frank T. and Sall, Amadou A.} }

Faye, Ousmane deposit-license Faye, Oumar 2011-06-16T08:49:09Z eng Nunes, Marcio R. T. Weidmann, Manfred Background<br />Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations.<br /><br />Objectives<br />To develop a real-time assay for YFV able to detect African and South American strains.<br /><br />Study design<br />Three short probe (14–18 nt) formats were compared and a plasmid-transcribed RNA standard was used to test the performance of the assays. Additionally the new TaqM1 enzyme was tested.<br /><br />Results<br />A locked nucleotide probe (LNA probe) performed best with an analytical sensitivity of 10 RNA molecules detected. 44 African and 10 South American strains were detectable. One South American strain from 1984 had a one-nucleotide deviation in the hybridisation sequence for which the LNA probe had to be adapted. Comparison of enzymes revealed that not all enzymes are suitable for LNA probes.<br /><br />Conclusion<br />The developed LNA probe based YFV real-time PCR performed best in an enzyme mix and less efficient using multifunctional enzymes. Weidmann, Manfred First publ. in: Journal of Clinical Virology 48 (2010), 3, pp. 187-192 2010 2011-06-16T08:49:09Z Hufert, Frank T. Vasconcelos, Pedro F. C. Nunes, Marcio R. T. Kranaster, Ramon Marx, Andreas Hufert, Frank T. Faye, Oumar Faye, Ousmane Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains Kranaster, Ramon Marx, Andreas Sall, Amadou A. Vasconcelos, Pedro F. C. Sall, Amadou A.

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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