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Analysis of a hybrid TATA box binding protein originating from mesophilic and thermophilic donor organisms

Analysis of a hybrid TATA box binding protein originating from mesophilic and thermophilic donor organisms

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KOPITZ, Annette, Jörg SOPPA, Karin HAUSER, 2010. Analysis of a hybrid TATA box binding protein originating from mesophilic and thermophilic donor organisms. In: Spectroscopy. 24(3-4), pp. 233-237. Available under: doi: 10.3233/SPE-2010-0434

@article{Kopitz2010Analy-12598, title={Analysis of a hybrid TATA box binding protein originating from mesophilic and thermophilic donor organisms}, year={2010}, doi={10.3233/SPE-2010-0434}, number={3-4}, volume={24}, journal={Spectroscopy}, pages={233--237}, author={Kopitz, Annette and Soppa, Jörg and Hauser, Karin} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/12598"> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:bibliographicCitation>First publ. in: Spectroscopy ; 24 (2010), 3-4. - S. 233-237</dcterms:bibliographicCitation> <dc:language>eng</dc:language> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-06-16T08:37:24Z</dcterms:available> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Hauser, Karin</dc:contributor> <dc:creator>Kopitz, Annette</dc:creator> <dcterms:issued>2010</dcterms:issued> <dc:rights>terms-of-use</dc:rights> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/12598"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:contributor>Soppa, Jörg</dc:contributor> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:contributor>Kopitz, Annette</dc:contributor> <dcterms:abstract xml:lang="eng">The TATA Box Binding Protein (TBP) is a 20 kD protein that is essential and universally conserved in eucarya and archaea. Especially among archaea, organisms can be found that live below 0°C as well as organisms that grow above 100°C. The archaeal TBPs show a high sequence identity and a similar structure consisting of α-helices and β-sheets that are arranged in a saddle-shape 2-symmetric fold. In previous studies, we have characterized the thermal stability of thermophilic and mesophilic archaeal TBPs by infrared spectroscopy and showed the correlation between the transition temperature (Tm) and the optimal growth temperature (OGT) of the respective donor organism. In this study, a “new” mutant TBP has been constructed, produced, purified and analyzed for a deeper understanding of the molecular mechanisms of thermoadaptation. The β-sheet part of the mutant consists of the TBP from Methanothermobacter thermoautotrophicus (OGT 65°C, MtTBP65) whose α-helices have been exchanged by those of Methanosarcina mazei (OGT 37°C, MmTBP37). The Hybrid-TBP irreversibly aggregates after thermal unfolding just like MmTBP37 and MtTBP65, but the Tm lies between that of MmTBP37 and MtTBP65 indicating that the interaction between the α-helical and β-sheet part of the TBP is crucial for the thermal stability. The temperature stability is probably encoded in the variable α-helices that interact with the highly conserved and DNA binding β-sheets.</dcterms:abstract> <dc:creator>Soppa, Jörg</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-06-16T08:37:24Z</dc:date> <dc:creator>Hauser, Karin</dc:creator> <dcterms:title>Analysis of a hybrid TATA box binding protein originating from mesophilic and thermophilic donor organisms</dcterms:title> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> </rdf:Description> </rdf:RDF>

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