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Caveolin limits membrane microdomain mobility and integrin-mediated uptake of fibronectin-binding pathogens

Caveolin limits membrane microdomain mobility and integrin-mediated uptake of fibronectin-binding pathogens

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HOFFMANN, Christine, Anne BERKING, Franziska AGERER, Alexander BUNTRU, Florian NESKE, G. Singh CHHATWAL, Knut OHLSEN, Christof HAUCK, 2010. Caveolin limits membrane microdomain mobility and integrin-mediated uptake of fibronectin-binding pathogens. In: Journal of Cell Science. 123(24), pp. 4280-4291. ISSN 0021-9533. eISSN 1477-9137

@article{Hoffmann2010-12-15Caveo-12570, title={Caveolin limits membrane microdomain mobility and integrin-mediated uptake of fibronectin-binding pathogens}, year={2010}, doi={10.1242/jcs.064006}, number={24}, volume={123}, issn={0021-9533}, journal={Journal of Cell Science}, pages={4280--4291}, author={Hoffmann, Christine and Berking, Anne and Agerer, Franziska and Buntru, Alexander and Neske, Florian and Chhatwal, G. Singh and Ohlsen, Knut and Hauck, Christof} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/12570"> <dc:contributor>Hoffmann, Christine</dc:contributor> <dc:creator>Chhatwal, G. Singh</dc:creator> <dcterms:bibliographicCitation>First publ. in: Journal of Cell Science 123 (2010), 24, pp. 4280-4291</dcterms:bibliographicCitation> <dc:creator>Ohlsen, Knut</dc:creator> <dcterms:abstract xml:lang="eng">Staphylococcus aureus, which is a leading cause of hospital-acquired infections, binds via fibronectin to integrin α5β1, a process that can promote host colonization in vivo. Integrin engagement induces actin cytoskeleton rearrangements that result in the uptake of S. aureus by non-professional phagocytic cells. Interestingly, we found that fibronectin-binding S. aureus trigger the redistribution of membrane microdomain components. In particular, ganglioside GM1 and GPI-linked proteins were recruited upon integrin β1 engagement, and disruption of membrane microdomains blocked bacterial internalization. Several membrane-microdomain-associated proteins, such as flotillin-1 and flotillin-2, as well as caveolin, were recruited to sites of bacterial attachment. Whereas dominant-negative versions of flotillin-2 did not affect bacterial attachment or internalization, cells deficient for caveolin-1 (Cav1–/–) showed increased uptake of S. aureus and other Fn-binding pathogens. Recruitment of membrane microdomains to cell-associated bacteria was unaltered in Cav1–/– cells. However, fluorescence recovery after photobleaching (FRAP) revealed an enhanced mobility of membrane-microdomain-associated proteins in the absence of caveolin-1. Enhanced membrane microdomain mobility and increased uptake of S. aureus was repressed by expression of wild-type caveolin-1, but not caveolin-1 G83S, which harbors a point mutation in the caveolin scaffolding domain. Similarly, chemical or physical stimulation of membrane fluidity led to increased uptake of S. aureus. These results highlight a crucial role for caveolin-1 in negative regulation of membrane microdomain mobility, thereby affecting endocytosis of bacteria-engaged integrins. This process might not only limit host cell invasion by integrin-binding bacterial pathogens, but might also be physiologically relevant for integrin-mediated cell adhesion.</dcterms:abstract> <dcterms:issued>2010-12-15</dcterms:issued> <dc:language>eng</dc:language> <dc:creator>Agerer, Franziska</dc:creator> <dc:creator>Neske, Florian</dc:creator> <dcterms:title>Caveolin limits membrane microdomain mobility and integrin-mediated uptake of fibronectin-binding pathogens</dcterms:title> <dc:contributor>Buntru, Alexander</dc:contributor> <dc:contributor>Chhatwal, G. Singh</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-06-21T08:25:41Z</dc:date> <dc:contributor>Berking, Anne</dc:contributor> <dc:contributor>Ohlsen, Knut</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/12570"/> <dc:creator>Berking, Anne</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103605204-4002607-1"/> <dc:rights>deposit-license</dc:rights> <dc:contributor>Neske, Florian</dc:contributor> <dc:creator>Hauck, Christof</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-12-30T23:25:13Z</dcterms:available> <dc:creator>Buntru, Alexander</dc:creator> <dc:contributor>Hauck, Christof</dc:contributor> <dc:contributor>Agerer, Franziska</dc:contributor> <dc:creator>Hoffmann, Christine</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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