KOPS - Das Institutionelle Repositorium der Universität Konstanz

Cyclic AMP increases endogenous granulocyte colony-stimulating factor formation in monocytes and THP-1 macrophages despite attenuated TNF-alpha formation

Cyclic AMP increases endogenous granulocyte colony-stimulating factor formation in monocytes and THP-1 macrophages despite attenuated TNF-alpha formation

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

HARENG, Lars, Thomas MEERGANS, Sonja von AULOCK, Hans-Dieter VOLK, Thomas HARTUNG, 2003. Cyclic AMP increases endogenous granulocyte colony-stimulating factor formation in monocytes and THP-1 macrophages despite attenuated TNF-alpha formation. In: European Journal of Immunology. 33(8), pp. 2287-2296. ISSN 0014-2980. eISSN 1521-4141

@article{Hareng2003Cycli-1230, title={Cyclic AMP increases endogenous granulocyte colony-stimulating factor formation in monocytes and THP-1 macrophages despite attenuated TNF-alpha formation}, year={2003}, doi={10.1002/eji.200323923}, number={8}, volume={33}, issn={0014-2980}, journal={European Journal of Immunology}, pages={2287--2296}, author={Hareng, Lars and Meergans, Thomas and Aulock, Sonja von and Volk, Hans-Dieter and Hartung, Thomas} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1230"> <dc:creator>Volk, Hans-Dieter</dc:creator> <dc:contributor>Aulock, Sonja von</dc:contributor> <dcterms:abstract xml:lang="eng">The cytokine granulocyte colony-stimulating factor (G-CSF) is in broad clinical use to treat neutropenia, and trials on its use in immunosuppressed conditions and infections are ongoing. To aply G-CSF effectively, it is crucial to understand the regulation and distribution of its endogenous formation. Since G-CSF release is mediated, at least in part, by TNF-α formation, we investigated whether drugs suppressing TNF-α also impair G-CSF production. Surprisingly, G-CSF formation was enhanced in lipopolysaccharide (LPS)-stimulated blood from a pentoxifylline-treated patient. In the presence of dibutyryl-cAMP, forskolin, tolafentrine or 3-isobutyl-1-methylxanthine, LPS-induced G-CSF formation was enhanced in THP-1 cells, primary monocytes and whole blood. Correspondingly,rp-8-bromo-cAMP suppressed LPS-induced G-CSF release. Addition of prostaglandin E2 enhanced and indomethacin suppressed G-CSF formation. Reporter gene studies showed that dibutyryl-cAMP enhanced LPS-induced G-CSF promoter activity, indicating a transcriptional up-regulation. Furthermore, disruption of a newly identified putative cAMP-responsive element (CRE) in the G-CSF promoter demonstrated the regulatory role for G-CSF gene transcription. In conclusion, endogenous G-CSF formation critically depends on both TNF-α and cyclooxygenase products, exerting effects via cAMP and the CREin the G-CSF promoter. This might have bearing for drug side effects, putative G-CSF mimetics and our understanding of G-CSF immunobiology.</dcterms:abstract> <dcterms:bibliographicCitation>First publ. in: European Journal of Immunology 33 (2003), 8, pp. 2287-2296</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:19Z</dcterms:available> <dc:creator>Hareng, Lars</dc:creator> <dcterms:title>Cyclic AMP increases endogenous granulocyte colony-stimulating factor formation in monocytes and THP-1 macrophages despite attenuated TNF-alpha formation</dcterms:title> <dc:creator>Aulock, Sonja von</dc:creator> <dc:contributor>Meergans, Thomas</dc:contributor> <dc:contributor>Hareng, Lars</dc:contributor> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:07:19Z</dc:date> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1230"/> <dc:rights>deposit-license</dc:rights> <dc:creator>Meergans, Thomas</dc:creator> <dc:contributor>Volk, Hans-Dieter</dc:contributor> <dcterms:issued>2003</dcterms:issued> <dc:creator>Hartung, Thomas</dc:creator> <dc:contributor>Hartung, Thomas</dc:contributor> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto