Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können kommenden Montag und Dienstag keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted next Monday and Tuesday.)

Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2

Cite This

Files in this item

Checksum: MD5:ba0acb85eb279b863d766657a267bb29

LANGHORST, Matthias F., Alexander REUTER, Friederike A. JAEGER, Frank M. WIPPICH, Georg LUXENHOFER, Helmut PLATTNER, Claudia STÜRMER, 2008. Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2. In: European Journal of Cell Biology. 87(4), pp. 211-226. ISSN 0171-9335. Available under: doi: 10.1016/j.ejcb.2007.12.001

@article{Langhorst2008-04Traff-1191, title={Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2}, year={2008}, doi={10.1016/j.ejcb.2007.12.001}, number={4}, volume={87}, issn={0171-9335}, journal={European Journal of Cell Biology}, pages={211--226}, author={Langhorst, Matthias F. and Reuter, Alexander and Jaeger, Friederike A. and Wippich, Frank M. and Luxenhofer, Georg and Plattner, Helmut and Stürmer, Claudia} }

<rdf:RDF xmlns:dcterms="" xmlns:dc="" xmlns:rdf="" xmlns:bibo="" xmlns:dspace="" xmlns:foaf="" xmlns:void="" xmlns:xsd="" > <rdf:Description rdf:about=""> <dc:contributor>Langhorst, Matthias F.</dc:contributor> <dc:language>eng</dc:language> <dcterms:rights rdf:resource=""/> <dcterms:isPartOf rdf:resource=""/> <dc:creator>Wippich, Frank M.</dc:creator> <dcterms:title>Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2</dcterms:title> <dc:creator>Luxenhofer, Georg</dc:creator> <bibo:uri rdf:resource=""/> <dspace:hasBitstream rdf:resource=""/> <dc:contributor>Wippich, Frank M.</dc:contributor> <dspace:isPartOfCollection rdf:resource=""/> <dc:creator>Stürmer, Claudia</dc:creator> <dc:contributor>Luxenhofer, Georg</dc:contributor> <dcterms:hasPart rdf:resource=""/> <dc:date rdf:datatype="">2011-03-23T09:06:44Z</dc:date> <dc:contributor>Stürmer, Claudia</dc:contributor> <dcterms:bibliographicCitation>First publ. in: European Journal of Cell Biology ; 87 (2008), 4. - S. 211-226</dcterms:bibliographicCitation> <dcterms:issued>2008-04</dcterms:issued> <dc:creator>Langhorst, Matthias F.</dc:creator> <dc:creator>Plattner, Helmut</dc:creator> <dc:creator>Jaeger, Friederike A.</dc:creator> <dc:creator>Reuter, Alexander</dc:creator> <dc:rights>terms-of-use</dc:rights> <dcterms:available rdf:datatype="">2011-03-23T09:06:44Z</dcterms:available> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Jaeger, Friederike A.</dc:contributor> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:contributor>Reuter, Alexander</dc:contributor> <dcterms:abstract xml:lang="eng">The reggie/flotillin proteins oligomerize and associate into clusters which form scaffolds for membrane microdomains. Besides their localization at the plasma membrane, the reggies/flotillins reside at various intracellular compartments; however, the trafficking pathways used by reggie-1/flotillin-2 remain unclear. Here, we show that trafficking of reggie-1/flotillin-2 is BFA sensitive and that deletion mutants of reggie-1/flotillin-2 accumulate in the Golgi complex in HeLa, Jurkat and PC12 cells, suggesting Golgi-dependent trafficking of reggie-1/flotillin-2. Using total internal reflection fluorescence microscopy, we observed fast cycling of reggie-1/flotillin-2-positive vesicles at the plasma membrane, which engaged in transient interactions with the plasma membrane only. Reggie-1/flotillin-2 cycling was independent of clathrin, but was inhibited by cholesterol depletion and microtubule disruption. Cycling of reggie-1/flotillin-2 was negatively correlated with cell cell contact formation but was stimulated by serum, epidermal growth factor and by cholesterol loading mediated by low density lipoproteins. However, reggie-1/flotillin-2 was neither involved in endocytosis of the epidermal growth factor itself nor in endocytosis of GPI-GFPs or the GPI-anchored cellular prion protein (PrPc). Reggie-2/flotillin-1 and stomatin-1 also exhibited cycling at the plasma membrane similar to reggie-1/flotillin-2, but these vesicles and microdomains only partially co-localized with reggie-2/flotillin-1. Thus, regulated vesicular cycling might be a general feature of SPFH protein-dependent trafficking.</dcterms:abstract> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Plattner etal.pdf 1822

This item appears in the following Collection(s)

Search KOPS


My Account