Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2
Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2
Date
2008
Authors
Langhorst, Matthias F.
Jaeger, Friederike A.
Wippich, Frank M.
Luxenhofer, Georg
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published in
European Journal of Cell Biology ; 87 (2008), 4. - pp. 211-226. - ISSN 0171-9335
Abstract
The reggie/flotillin proteins oligomerize and associate into clusters which form scaffolds for membrane microdomains. Besides their localization at the plasma membrane, the reggies/flotillins reside at various intracellular compartments; however, the trafficking pathways used by reggie-1/flotillin-2 remain unclear. Here, we show that trafficking of reggie-1/flotillin-2 is BFA sensitive and that deletion mutants of reggie-1/flotillin-2 accumulate in the Golgi complex in HeLa, Jurkat and PC12 cells, suggesting Golgi-dependent trafficking of reggie-1/flotillin-2. Using total internal reflection fluorescence microscopy, we observed fast cycling of reggie-1/flotillin-2-positive vesicles at the plasma membrane, which engaged in transient interactions with the plasma membrane only. Reggie-1/flotillin-2 cycling was independent of clathrin, but was inhibited by cholesterol depletion and microtubule disruption. Cycling of reggie-1/flotillin-2 was negatively correlated with cell cell contact formation but was stimulated by serum, epidermal growth factor and by cholesterol loading mediated by low density lipoproteins. However, reggie-1/flotillin-2 was neither involved in endocytosis of the epidermal growth factor itself nor in endocytosis of GPI-GFPs or the GPI-anchored cellular prion protein (PrPc). Reggie-2/flotillin-1 and stomatin-1 also exhibited cycling at the plasma membrane similar to reggie-1/flotillin-2, but these vesicles and microdomains only partially co-localized with reggie-2/flotillin-1. Thus, regulated vesicular cycling might be a general feature of SPFH protein-dependent trafficking.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Reggie/flotillin,Membrane trafficking,TIRF microscopy,Endocytosis,Endocytic cycling
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
LANGHORST, Matthias F., Alexander REUTER, Friederike A. JAEGER, Frank M. WIPPICH, Georg LUXENHOFER, Helmut PLATTNER, Claudia STÜRMER, 2008. Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2. In: European Journal of Cell Biology. 87(4), pp. 211-226. ISSN 0171-9335. Available under: doi: 10.1016/j.ejcb.2007.12.001BibTex
@article{Langhorst2008-04Traff-1191, year={2008}, doi={10.1016/j.ejcb.2007.12.001}, title={Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2}, number={4}, volume={87}, issn={0171-9335}, journal={European Journal of Cell Biology}, pages={211--226}, author={Langhorst, Matthias F. and Reuter, Alexander and Jaeger, Friederike A. and Wippich, Frank M. and Luxenhofer, Georg and Plattner, Helmut and Stürmer, Claudia} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/1191"> <dc:contributor>Stürmer, Claudia</dc:contributor> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:44Z</dc:date> <dc:creator>Langhorst, Matthias F.</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:creator>Reuter, Alexander</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:issued>2008-04</dcterms:issued> <dcterms:abstract xml:lang="eng">The reggie/flotillin proteins oligomerize and associate into clusters which form scaffolds for membrane microdomains. Besides their localization at the plasma membrane, the reggies/flotillins reside at various intracellular compartments; however, the trafficking pathways used by reggie-1/flotillin-2 remain unclear. Here, we show that trafficking of reggie-1/flotillin-2 is BFA sensitive and that deletion mutants of reggie-1/flotillin-2 accumulate in the Golgi complex in HeLa, Jurkat and PC12 cells, suggesting Golgi-dependent trafficking of reggie-1/flotillin-2. Using total internal reflection fluorescence microscopy, we observed fast cycling of reggie-1/flotillin-2-positive vesicles at the plasma membrane, which engaged in transient interactions with the plasma membrane only. Reggie-1/flotillin-2 cycling was independent of clathrin, but was inhibited by cholesterol depletion and microtubule disruption. Cycling of reggie-1/flotillin-2 was negatively correlated with cell cell contact formation but was stimulated by serum, epidermal growth factor and by cholesterol loading mediated by low density lipoproteins. However, reggie-1/flotillin-2 was neither involved in endocytosis of the epidermal growth factor itself nor in endocytosis of GPI-GFPs or the GPI-anchored cellular prion protein (PrPc). Reggie-2/flotillin-1 and stomatin-1 also exhibited cycling at the plasma membrane similar to reggie-1/flotillin-2, but these vesicles and microdomains only partially co-localized with reggie-2/flotillin-1. Thus, regulated vesicular cycling might be a general feature of SPFH protein-dependent trafficking.</dcterms:abstract> <dc:contributor>Langhorst, Matthias F.</dc:contributor> <dc:creator>Wippich, Frank M.</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1191"/> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:contributor>Luxenhofer, Georg</dc:contributor> <dcterms:bibliographicCitation>First publ. in: European Journal of Cell Biology ; 87 (2008), 4. - S. 211-226</dcterms:bibliographicCitation> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/1191/1/Plattner%20etal.pdf"/> <dc:contributor>Wippich, Frank M.</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/1191/1/Plattner%20etal.pdf"/> <dc:creator>Luxenhofer, Georg</dc:creator> <dc:contributor>Jaeger, Friederike A.</dc:contributor> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:title>Trafficking of the microdomain scaffolding protein reggie-1/flotillin-2</dcterms:title> <dc:creator>Stürmer, Claudia</dc:creator> <dc:language>eng</dc:language> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Reuter, Alexander</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:44Z</dcterms:available> <dc:creator>Plattner, Helmut</dc:creator> <dc:creator>Jaeger, Friederike A.</dc:creator> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes