KOPS - Das Institutionelle Repositorium der Universität Konstanz

Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36

Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

NILSEN, Nadra J., Susanne DEININGER, Unni NONSTAD, Frode SKJELDAL, Harald HUSEBYE, Dmitrii RODIONOV, Sonja von AULOCK, Thomas HARTUNG, Egil LIEN, Oddmund BAKKE, Terje ESPEVIK, 2008. Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36. In: Journal of Leukocyte Biology. 84(1), pp. 280-291. ISSN 0741-5400

@article{Nilsen2008Cellu-1175, title={Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36}, year={2008}, doi={10.1189/jlb.0907656}, number={1}, volume={84}, issn={0741-5400}, journal={Journal of Leukocyte Biology}, pages={280--291}, author={Nilsen, Nadra J. and Deininger, Susanne and Nonstad, Unni and Skjeldal, Frode and Husebye, Harald and Rodionov, Dmitrii and Aulock, Sonja von and Hartung, Thomas and Lien, Egil and Bakke, Oddmund and Espevik, Terje} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1175"> <dc:contributor>Husebye, Harald</dc:contributor> <dc:creator>Espevik, Terje</dc:creator> <dcterms:abstract xml:lang="eng">Lipoteichoic acid (LTA) is a central inducer of inflammatory responses caused by Gram-positive bacteria, such as Staphylococcus aureus, via activation of TLR2. Localization of TLR2 in relation to its coreceptors may be important for function. This study explores the signaling, uptake, and trafficking pattern of LTA in relation to expression of TLR2 and its coreceptors CD36 and CD14 in human monocytes. We found TLR2 expressed in early endosomes, late endosomes/lysosomes, and in Rab-11-positive compartments but not in the Golgi apparatus or endoplasmic reticulum (ER). Rapid internalization of fluorescently labeled LTA was observed in human monocytes, colocalizing with markers for early and late endosomes, lysosomes, ER, and Golgi network. Blocking CD14 and CD36 with antibodies inhibited LTA binding and LTA-induced TNF release from monocytes, emphasizing an important role for both molecules as coreceptors for TLR2. Importantly, blocking CD36 did not affect TNF release induced by N-palmitoyl-S-[2,3-bis(palmitoyloxy)-(2R,S)-propyl]-(R)-cysteinyl-seryl-(lysyl)3-lysine or LPS. Expression of CD14 markedly enhanced LTA binding to the plasma membrane and also enhanced NF-{kappa}B activation. LTA internalization, but not NF-{kappa}B activation, was inhibited in Dynamin-I K44A dominant-negative transfectants, suggesting that LTA is internalized by receptor-mediated endocytosis but that internalization is not required for signaling. In fact, immobilizing LTA and thereby inhibiting internalization resulted in enhanced TNF release from monocytes. Our results suggest that LTA signaling preferentially occurs at the plasma membrane, is independent of internalization, and is facilitated by CD36 and CD14 as coreceptors for TLR2.</dcterms:abstract> <dc:creator>Skjeldal, Frode</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:contributor>Nilsen, Nadra J.</dc:contributor> <dc:language>eng</dc:language> <dc:contributor>Lien, Egil</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:38Z</dc:date> <dc:contributor>Aulock, Sonja von</dc:contributor> <dc:creator>Aulock, Sonja von</dc:creator> <dc:contributor>Rodionov, Dmitrii</dc:contributor> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1175"/> <dc:contributor>Deininger, Susanne</dc:contributor> <dc:creator>Bakke, Oddmund</dc:creator> <dc:contributor>Hartung, Thomas</dc:contributor> <dc:creator>Hartung, Thomas</dc:creator> <dc:creator>Deininger, Susanne</dc:creator> <dc:creator>Lien, Egil</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:38Z</dcterms:available> <dc:contributor>Skjeldal, Frode</dc:contributor> <dc:creator>Nonstad, Unni</dc:creator> <dcterms:issued>2008</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:contributor>Bakke, Oddmund</dc:contributor> <dc:creator>Nilsen, Nadra J.</dc:creator> <dc:creator>Husebye, Harald</dc:creator> <dc:contributor>Nonstad, Unni</dc:contributor> <dcterms:bibliographicCitation>Publ. in: Journal of Leukocyte Biology 84 (2008), 1, pp. 280-291</dcterms:bibliographicCitation> <dc:contributor>Espevik, Terje</dc:contributor> <dc:creator>Rodionov, Dmitrii</dc:creator> <dcterms:title>Cellular trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36</dcterms:title> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto