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Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic Di-GMP levels, and enhanced dispersal

Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic Di-GMP levels, and enhanced dispersal

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BARRAUD, Nicolas, David SCHLEHECK, Janosch KLEBENSBERGER, Jeremy S. WEBB, Daniel J. HASSETT, Scott A. RICE, Staffan KJELLEBERG, 2009. Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic Di-GMP levels, and enhanced dispersal. In: Journal of Bacteriology. 191(23), pp. 7333-7342. ISSN 0021-9193. eISSN 1098-5530

@article{Barraud2009Nitri-1164, title={Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic Di-GMP levels, and enhanced dispersal}, year={2009}, doi={10.1128/JB.00975-09}, number={23}, volume={191}, issn={0021-9193}, journal={Journal of Bacteriology}, pages={7333--7342}, author={Barraud, Nicolas and Schleheck, David and Klebensberger, Janosch and Webb, Jeremy S. and Hassett, Daniel J. and Rice, Scott A. and Kjelleberg, Staffan} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1164"> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:contributor>Hassett, Daniel J.</dc:contributor> <dc:contributor>Rice, Scott A.</dc:contributor> <dc:contributor>Schleheck, David</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:34Z</dc:date> <dc:creator>Schleheck, David</dc:creator> <dc:creator>Hassett, Daniel J.</dc:creator> <dc:rights>deposit-license</dc:rights> <dc:creator>Rice, Scott A.</dc:creator> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:34Z</dcterms:available> <dc:creator>Klebensberger, Janosch</dc:creator> <dc:contributor>Kjelleberg, Staffan</dc:contributor> <dc:creator>Webb, Jeremy S.</dc:creator> <dc:creator>Barraud, Nicolas</dc:creator> <dc:contributor>Klebensberger, Janosch</dc:contributor> <dcterms:issued>2009</dcterms:issued> <dc:contributor>Webb, Jeremy S.</dc:contributor> <dcterms:abstract xml:lang="eng">Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa, at low, non-toxic concentrations (Barraud, N., D. J. Hassett, S. H. Hwang, S. A. Rice, S. Kjelleberg, and J. S. Webb. 2006. J. Bacteriol. 188:7344 7353). NO was further shown to increase cell motility and susceptibility to antimicrobials. Recently, numerous studies revealed that increased degradation of the secondary messenger cyclic diguanosine-5'-monophosphate (c-di-GMP) by specific phosphodiesterases (PDEs) triggers a planktonic mode of growth in eubacteria. In this study, the potential link between NO and c-di-GMP signaling was investigated by performing (i) PDE inhibitor studies, (ii) enzymatic assays to measure PDE activity, and (iii) direct quantification of intracellular c-di-GMP levels. The results suggest a role for c-di-GMP signaling in triggering the biofilm dispersal event induced by NO, as dispersal requires PDE activity and addition of NO stimulates PDE and induces the concomitant decrease in intracellular c-di-GMP levels in P. aeruginosa. Furthermore, gene expression studies indicated global responses to low, non-toxic levels of NO in P. aeruginosa biofilms, including upregulation of genes involved in motility and energy metabolism and downregulation of adhesins and virulence factors. Finally, site-directed mutagenesis of candidate genes and physiological characterization of the corresponding mutant strains uncovered that the chemotaxis transducer BdlA is involved in the biofilm dispersal response induced by NO.</dcterms:abstract> <dc:contributor>Barraud, Nicolas</dc:contributor> <dc:language>eng</dc:language> <dcterms:bibliographicCitation>Publ. in: Journal of Bacteriology 191 (2009), 23, pp. 7333-7342</dcterms:bibliographicCitation> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1164"/> <dc:creator>Kjelleberg, Staffan</dc:creator> <dcterms:title>Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic Di-GMP levels, and enhanced dispersal</dcterms:title> </rdf:Description> </rdf:RDF>

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