Type of Publication: | Journal article |
Author: | Ruff, Jürgen; Smits, Theo H. M.; Cook, Alasdair M.; Schleheck, David |
Year of publication: | 2010 |
Published in: | Microbiological Research ; 165 (2010), 4. - pp. 288-299. - ISSN 0944-5013. - eISSN 1618-0623 |
Pubmed ID: | 19577910 |
DOI (citable link): | https://dx.doi.org/10.1016/j.micres.2009.05.006 |
Summary: |
Alcaligenes sp. strain O-1 inducibly deaminates 2-aminobenzenesulfonate (ABS) via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway (Scm). The complete pathway is encoded on the 180-kb plasmid pSAH, 20 kb of which was sequenced. Twenty open reading frames (ORFs) were detected. Two clusters (abs and scm) with degradative genes were surrounded by several transposon-related ORFs. The six genes of the abs cluster were shown to be co-transcribed, and contained the genes for two characterised subunits of the oxygenase component of the ABS-dioxygenase system, and genes putatively encoding ABS-transport functions with similarities to (a) an ABC-type transporter system and (b) a putative major facilitator superfamily transporter. No gene encoding the reductase for the oxygenase system was present in the abs gene cluster, but a candidate gene was found in the scm cluster. The seven-gene scm cluster was also transcribed as single polycistronic message. Functions could be attributed to the gene products, but one enzyme, which was shown to be present, 2-hydroxymuconate isomerase, was not encoded in the scm cluster. No transcriptional regulator was found. This genetic information on the degradation of ABS in strain O-1 provides another example of both split operons and dispersed pathway genes.
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Subject (DDC): | 570 Biosciences, Biology |
Keywords: | 2-aminobenzenesulfonate, 3-sulfocatechol, Degradative pathway, Oxygenase, Meta-cleavage |
Bibliography of Konstanz: | Yes |
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RUFF, Jürgen, Theo H. M. SMITS, Alasdair M. COOK, David SCHLEHECK, 2010. Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1. In: Microbiological Research. 165(4), pp. 288-299. ISSN 0944-5013. eISSN 1618-0623. Available under: doi: 10.1016/j.micres.2009.05.006
@article{Ruff2010Ident-1142, title={Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1}, year={2010}, doi={10.1016/j.micres.2009.05.006}, number={4}, volume={165}, issn={0944-5013}, journal={Microbiological Research}, pages={288--299}, author={Ruff, Jürgen and Smits, Theo H. M. and Cook, Alasdair M. and Schleheck, David} }
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