KOPS - Das Institutionelle Repositorium der Universität Konstanz

Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1

Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

RUFF, Jürgen, Theo H. M. SMITS, Alasdair M. COOK, David SCHLEHECK, 2010. Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1. In: Microbiological Research. 165(4), pp. 288-299. ISSN 0944-5013. eISSN 1618-0623

@article{Ruff2010Ident-1142, title={Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1}, year={2010}, doi={10.1016/j.micres.2009.05.006}, number={4}, volume={165}, issn={0944-5013}, journal={Microbiological Research}, pages={288--299}, author={Ruff, Jürgen and Smits, Theo H. M. and Cook, Alasdair M. and Schleheck, David} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1142"> <dc:rights>deposit-license</dc:rights> <dc:contributor>Cook, Alasdair M.</dc:contributor> <dc:creator>Schleheck, David</dc:creator> <dc:language>eng</dc:language> <dc:creator>Smits, Theo H. M.</dc:creator> <dcterms:title>Identification of two vicinal operons for the degradation of 2-aminobenzenesulfonate encoded on plasmid pSAH in Alcaligenes sp. strain O-1</dcterms:title> <dc:contributor>Schleheck, David</dc:contributor> <dcterms:abstract xml:lang="eng">Alcaligenes sp. strain O-1 inducibly deaminates 2-aminobenzenesulfonate (ABS) via dioxygenation to 3-sulfocatechol, which is desulfonated during meta ring-cleavage to yield 2-hydroxymuconate. This intermediate is transformed through the oxalocrotonate-branch of the sulfocatechol meta-pathway (Scm). The complete pathway is encoded on the 180-kb plasmid pSAH, 20 kb of which was sequenced. Twenty open reading frames (ORFs) were detected. Two clusters (abs and scm) with degradative genes were surrounded by several transposon-related ORFs. The six genes of the abs cluster were shown to be co-transcribed, and contained the genes for two characterised subunits of the oxygenase component of the ABS-dioxygenase system, and genes putatively encoding ABS-transport functions with similarities to (a) an ABC-type transporter system and (b) a putative major facilitator superfamily transporter. No gene encoding the reductase for the oxygenase system was present in the abs gene cluster, but a candidate gene was found in the scm cluster. The seven-gene scm cluster was also transcribed as single polycistronic message. Functions could be attributed to the gene products, but one enzyme, which was shown to be present, 2-hydroxymuconate isomerase, was not encoded in the scm cluster. No transcriptional regulator was found. This genetic information on the degradation of ABS in strain O-1 provides another example of both split operons and dispersed pathway genes.</dcterms:abstract> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1142"/> <dcterms:issued>2010</dcterms:issued> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:25Z</dc:date> <dcterms:bibliographicCitation>Publ. in: Microbiological Research 165 (2010), 4, pp. 288-299</dcterms:bibliographicCitation> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:25Z</dcterms:available> <dc:contributor>Smits, Theo H. M.</dc:contributor> <dc:creator>Ruff, Jürgen</dc:creator> <dc:contributor>Ruff, Jürgen</dc:contributor> <dc:creator>Cook, Alasdair M.</dc:creator> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto