Zebrafish Neurolin-a and -b, Orthologs of ALCAM, are Involved in Retinal Ganglion Cell Differentiation and Retinal Axon Pathfinding

Diekmann, Heike; Stürmer, Claudia

Zebrafish Neurolin-a and -b, Orthologs of ALCAM, are Involved in Retinal Ganglion Cell Differentiation and Retinal Axon Pathfinding

Files

Stuermer_111801.pdf(3.49 MB)

Date

2009

Authors

Diekmann, Heike

Stürmer, Claudia

Publication type

Journal article

Published in

Journal of Comparative Neurology ; 513 (2009), 1. - pp. 38-50. - ISSN 0021-9967. - eISSN 1096-9861

Abstract

Neurolin-a and Neurolin-b (also called alcam and nlcam, respectively) are zebrafish orthologs of human ALCAM, an adhesion protein of the immunoglobulin superfamily with functions in axon growth and guidance. Within the developing zebrafish retina, onset and progression of Neurolin-a expression parallels the pattern of retinal ganglion cell (RGC) differentiation. By using a morpholino-based knockdown approach, we show that Neurolin-a (but not Neurolin-b) is necessary for a crucial step in RGC differentiation. Without Neurolin-a, a large proportion of RGCs fail to develop, and RGC axons are absent or reduced in number. Subsequently, Neurolin-a is required for RGC survival and for the differentiation of all other retinal neurons. Neurolin-b is expressed later in well-differentiated RGCs and is required for RGC axon pathfinding. Without Neurolin-b, RGC axons grow in highly aberrant routes along the optic tract and/or fail to reach the optic tectum. Thus, the zebrafish Neurolin paralogs are involved in distinct steps of retinotectal development.

Subject (DDC)

570 Biosciences, Biology

Keywords

adhesion molecule,dm-grasp,retinotectal projection,surface glycoprotein,fate determination,sonic hedgehog,immunoglobulin superfamily

Cite This

ISO 690

DIEKMANN, Heike, Claudia STÜRMER, 2009. Zebrafish Neurolin-a and -b, Orthologs of ALCAM, are Involved in Retinal Ganglion Cell Differentiation and Retinal Axon Pathfinding. In: Journal of Comparative Neurology. 513(1), pp. 38-50. ISSN 0021-9967. eISSN 1096-9861. Available under: doi: 10.1002/cne.21928

BibTex

@article{Diekmann2009Zebra-1139,
  year={2009},
  doi={10.1002/cne.21928},
  title={Zebrafish Neurolin-a and -b, Orthologs of ALCAM, are Involved in Retinal Ganglion Cell Differentiation and Retinal Axon Pathfinding},
  number={1},
  volume={513},
  issn={0021-9967},
  journal={Journal of Comparative Neurology},
  pages={38--50},
  author={Diekmann, Heike and Stürmer, Claudia}
}

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Bibliography of Konstanz

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