KOPS - Das Institutionelle Repositorium der Universität Konstanz

Reggies/flotillins regulate retinal axon regeneration in the zebrafish optic nerve and differentiation of hippocampal and N2a neurons

Reggies/flotillins regulate retinal axon regeneration in the zebrafish optic nerve and differentiation of hippocampal and N2a neurons

Zitieren

Dateien zu dieser Ressource

Prüfsumme: MD5:252a2cc354a90c16fc81e35e1189026f

MUNDERLOH, Christina, Gonzalo SOLIS PADILLA, Vsevolod BODRIKOV, Friederike A. JAEGER, Marianne F. WIECHERS, Edward MÁLAGA-TRILLO, Claudia STÜRMER, 2009. Reggies/flotillins regulate retinal axon regeneration in the zebrafish optic nerve and differentiation of hippocampal and N2a neurons. In: The Journal of Neuroscience. 29(20), pp. 6607-6615. ISSN 0270-6474. eISSN 1529-2401

@article{Munderloh2009Reggi-1116, title={Reggies/flotillins regulate retinal axon regeneration in the zebrafish optic nerve and differentiation of hippocampal and N2a neurons}, year={2009}, doi={10.1523/JNEUROSCI.0870-09.2009}, number={20}, volume={29}, issn={0270-6474}, journal={The Journal of Neuroscience}, pages={6607--6615}, author={Munderloh, Christina and Solis Padilla, Gonzalo and Bodrikov, Vsevolod and Jaeger, Friederike A. and Wiechers, Marianne F. and Málaga-Trillo, Edward and Stürmer, Claudia} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1116"> <dc:contributor>Wiechers, Marianne F.</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:15Z</dc:date> <dc:creator>Munderloh, Christina</dc:creator> <dc:creator>Wiechers, Marianne F.</dc:creator> <dc:contributor>Jaeger, Friederike A.</dc:contributor> <dc:contributor>Bodrikov, Vsevolod</dc:contributor> <dc:rights>deposit-license</dc:rights> <dcterms:abstract xml:lang="eng">The reggies/flotillins proteins upregulated during axon regeneration in retinal ganglion cells (RGCs) are scaffolding proteins of microdomains and involved in neuronal differentiation. Here, we show that reggies regulate axon regeneration in zebrafish (ZF) after optic nerve section (ONS) in vivo as well as axon/neurite extension in hippocampal and N2a neurons in vitro through signal transduction molecules modulating actin dynamics. ZF reggie-1a, -2a, and -2b downregulation by reggie-specific morpholino (Mo) antisense oligonucleotides directly after ONS significantly reduced ZF RGC axon regeneration: RGC axons from reggie Mo retinas were markedly reduced. Moreover, the number of axon-regenerating RGCs, identified by insertion of A488-coupled dextran, decreased by 69% in retinas 7 d after Mo application. At 10 and 14 d, RGCs decreased by 53 and 33%, respectively, in correlation with the gradual inactivation of the Mos. siRNA-mediated knockdown of reggie-1 and -2 inhibited the differentiation and axon/neurite extension in hippocampal and N2a neurons. N2a cells had significantly shorter filopodia, more cells had lamellipodia and fewer neurites, defects which were rescued by a reggie-1 construct without siRNA-binding sites. Furthermore, reggie knockdown strongly perturbed the balanced activation of the Rho family GTPases Rac1, RhoA, and cdc42, influenced the phosphorylation of cortactin and cofilin, the formation of the N-WASP, cortactin and Arp3 complex, and affected p38, Ras, ERK1/2 (extracellular signal-regulated kinases 1 and 2), and focal adhesion kinase activation. Thus, as suggested by their prominent re-expression after lesion, the reggies represent neuron-intrinsic factors for axon outgrowth and regeneration, being crucial for the coordinated assembly of signaling complexes regulating cytoskeletal remodeling.</dcterms:abstract> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:15Z</dcterms:available> <dc:language>eng</dc:language> <dcterms:issued>2009</dcterms:issued> <dc:creator>Jaeger, Friederike A.</dc:creator> <dc:creator>Bodrikov, Vsevolod</dc:creator> <dc:creator>Solis Padilla, Gonzalo</dc:creator> <dcterms:bibliographicCitation>The Journal of Neuroscience ; 29 (2009), 20. - S. 6607-6615</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:contributor>Málaga-Trillo, Edward</dc:contributor> <dcterms:title>Reggies/flotillins regulate retinal axon regeneration in the zebrafish optic nerve and differentiation of hippocampal and N2a neurons</dcterms:title> <dc:creator>Málaga-Trillo, Edward</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1116"/> <dc:contributor>Solis Padilla, Gonzalo</dc:contributor> <dc:contributor>Stürmer, Claudia</dc:contributor> <dc:contributor>Munderloh, Christina</dc:contributor> <dc:creator>Stürmer, Claudia</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

Stuermer_111793.pdf 80

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto