KOPS - Das Institutionelle Repositorium der Universität Konstanz

Binding Epitopes and Interaction Structure of the Neuroprotective Protease Inhibitor Cystatin C with beta-Amyloid Revealed by Proteolytic Excision Mass Spectrometry and Molecular Docking Simulation

Binding Epitopes and Interaction Structure of the Neuroprotective Protease Inhibitor Cystatin C with beta-Amyloid Revealed by Proteolytic Excision Mass Spectrometry and Molecular Docking Simulation

Zitieren

Dateien zu dieser Ressource

Dateien Größe Format Anzeige

Zu diesem Dokument gibt es keine Dateien.

JUSZCZYK, Paulina, Gabriela PARASCHIV, Aneta SZYMANSKA, Aneta S. KOLODZIEJCZYK, Sylwia RODZIEWICZ-MOTOWIDLO, Zbigniew GRZONKA, Michael PRZYBYLSKI, 2009. Binding Epitopes and Interaction Structure of the Neuroprotective Protease Inhibitor Cystatin C with beta-Amyloid Revealed by Proteolytic Excision Mass Spectrometry and Molecular Docking Simulation. In: Journal of Medicinal Chemistry. 52(8), pp. 2420-2428. ISSN 0022-2623. eISSN 1520-4804

@article{Juszczyk2009Bindi-1088, title={Binding Epitopes and Interaction Structure of the Neuroprotective Protease Inhibitor Cystatin C with beta-Amyloid Revealed by Proteolytic Excision Mass Spectrometry and Molecular Docking Simulation}, year={2009}, doi={10.1021/jm801115e}, number={8}, volume={52}, issn={0022-2623}, journal={Journal of Medicinal Chemistry}, pages={2420--2428}, author={Juszczyk, Paulina and Paraschiv, Gabriela and Szymanska, Aneta and Kolodziejczyk, Aneta S. and Rodziewicz-Motowidlo, Sylwia and Grzonka, Zbigniew and Przybylski, Michael} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1088"> <dc:contributor>Paraschiv, Gabriela</dc:contributor> <dcterms:bibliographicCitation>Publ. in: Journal of Medicinal Chemistry 52 (2009), 8, pp. 2420-2428</dcterms:bibliographicCitation> <dc:creator>Kolodziejczyk, Aneta S.</dc:creator> <dcterms:abstract>Human cystatin C (HCC) is a protease inhibitor with a propensity to form beta-amyloid (Abeta)-like fibrils and to coassociate with amyloidogenic proteins. Recently, a specific interaction between HCC and Abeta has been found. Here, we report the identification of the Abeta and HCC binding epitopes in the Abeta-HCC complex, using a combination of selective proteolytic excision and high resolution mass spectrometry. Proteolytic excision of Abeta(1-40) on sepharose-immobilized HCC and MALDI-MS identified the epitope Abeta(17-28). On immobilized Abeta(1-40), affinity MS of HCC fragments identified a specific C-terminal epitope, HCC(101-117). Binding specificities of both epitopes were ascertained by ELISA and surface plasmon resonance and by direct electrospray MS of the HCC-Abeta epitope peptide complexes. A structure model of the HCC-Abeta complex by molecular docking simulation showed full agreement with the identified Abeta and HCC epitopes. Inhibition studies in vitro revealed Abeta-fibril inhibiting activity of the HCC(101-117)-epitope. The Abeta-HCC interacting epitopes provide lead structures of neuroprotective inhibitors for AD and HCC amyloidosis therapy.</dcterms:abstract> <dc:creator>Paraschiv, Gabriela</dc:creator> <dc:contributor>Szymanska, Aneta</dc:contributor> <dc:creator>Rodziewicz-Motowidlo, Sylwia</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:creator>Szymanska, Aneta</dc:creator> <dcterms:title>Binding Epitopes and Interaction Structure of the Neuroprotective Protease Inhibitor Cystatin C with beta-Amyloid Revealed by Proteolytic Excision Mass Spectrometry and Molecular Docking Simulation</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-22T17:55:10Z</dcterms:available> <dc:contributor>Przybylski, Michael</dc:contributor> <dc:contributor>Grzonka, Zbigniew</dc:contributor> <dc:creator>Przybylski, Michael</dc:creator> <dc:contributor>Kolodziejczyk, Aneta S.</dc:contributor> <dc:contributor>Rodziewicz-Motowidlo, Sylwia</dc:contributor> <dc:contributor>Juszczyk, Paulina</dc:contributor> <dc:rights>deposit-license</dc:rights> <dc:language>eng</dc:language> <dc:creator>Juszczyk, Paulina</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1088"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-22T17:55:10Z</dc:date> <dc:creator>Grzonka, Zbigniew</dc:creator> <dcterms:issued>2009</dcterms:issued> </rdf:Description> </rdf:RDF>

Das Dokument erscheint in:

KOPS Suche


Stöbern

Mein Benutzerkonto