Type of Publication: | Journal article |
Author: | Marquardt, Andreas; Bernevic, Bogdan; Przybylski, Michael |
Year of publication: | 2007 |
Published in: | Journal of Peptide Science ; 13 (2007), 12. - pp. 803-810. - ISSN 1075-2617. - eISSN 1099-1387 |
Pubmed ID: | 17918767 |
DOI (citable link): | https://dx.doi.org/10.1002/psc.902 |
Summary: |
A cyclic disulfide heptadecapeptide (TIP17ox; 2) derived from the lectin-like 17-amino acid domain of human tumor necrosis factor-α [TNF-α (100-116)] was synthesised and demonstrated to bind specifically to N,N-diacetylchitobiose, a disaccharide present in many glycan structures of glycoproteins. Although the TIP domain forms a loop structure in the native TNF-α protein, we show in this study by high-resolution ESI-FTICR mass spectrometry that a homologous linear heptadecapeptide (TIP17rd; 1) binds with comparable affinity to chitobiose, suggesting that cyclisation is not essential for carbohydrate binding. ESI-FTICR-MS was used as an efficient tool for the direct molecular characterisation of TIP peptide-carbohydrate complexes. The specific binding of the TNF-TIP domain to chitobiose and other carbohydrate motifs in glycoproteins may explain the high proteolytic stability of these peptides in biological fluids. A considerably higher proteolytic stability in human plasma was found by mass spectrometric analysis for the cyclic TIP peptide 2, compared to the linear peptide 1. Furthermore, affinity-proteomics studies using immobilised cyclic TIP peptide 2 provided the identification of specific interacting glycoproteins in plasma.
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Subject (DDC): | 540 Chemistry |
Keywords: | TNF-alpha, lectin-like peptide domain, cyclic TIP peptide-carbohydrate complexes, ESI-FTICR mass spectrometry, proteolytic stability |
Bibliography of Konstanz: | Yes |
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MARQUARDT, Andreas, Bogdan BERNEVIC, Michael PRZYBYLSKI, 2007. Identification, affinity characterisation and biological interactions of lectin-like peptide-carbohydrate complexes derived from human TNF-alpha using high-resolution mass spectrometry. In: Journal of Peptide Science. 13(12), pp. 803-810. ISSN 1075-2617. eISSN 1099-1387. Available under: doi: 10.1002/psc.902
@article{Marquardt2007Ident-1044, title={Identification, affinity characterisation and biological interactions of lectin-like peptide-carbohydrate complexes derived from human TNF-alpha using high-resolution mass spectrometry}, year={2007}, doi={10.1002/psc.902}, number={12}, volume={13}, issn={1075-2617}, journal={Journal of Peptide Science}, pages={803--810}, author={Marquardt, Andreas and Bernevic, Bogdan and Przybylski, Michael} }
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