@article{Marquardt2007Ident-1044,
  year={2007},
  doi={10.1002/psc.902},
  title={Identification, affinity characterisation and biological interactions of lectin-like peptide-carbohydrate complexes derived from human TNF-alpha using high-resolution mass spectrometry},
  number={12},
  volume={13},
  issn={1075-2617},
  journal={Journal of Peptide Science},
  pages={803--810},
  author={Marquardt, Andreas and Bernevic, Bogdan and Przybylski, Michael}
}

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    <dcterms:abstract xml:lang="eng">A cyclic disulfide heptadecapeptide (TIP17ox; 2) derived from the lectin-like 17-amino acid domain of human tumor necrosis factor-α [TNF-α (100-116)] was synthesised and demonstrated to bind specifically to N,N-diacetylchitobiose, a disaccharide present in many glycan structures of glycoproteins. Although the TIP domain forms a loop structure in the native TNF-α protein, we show in this study by high-resolution ESI-FTICR mass spectrometry that a homologous linear heptadecapeptide (TIP17rd; 1) binds with comparable affinity to chitobiose, suggesting that cyclisation is not essential for carbohydrate binding. ESI-FTICR-MS was used as an efficient tool for the direct molecular characterisation of TIP peptide-carbohydrate complexes. The specific binding of the TNF-TIP domain to chitobiose and other carbohydrate motifs in glycoproteins may explain the high proteolytic stability of these peptides in biological fluids. A considerably higher proteolytic stability in human plasma was found by mass spectrometric analysis for the cyclic TIP peptide 2, compared to the linear peptide 1. Furthermore, affinity-proteomics studies using immobilised cyclic TIP peptide 2 provided the identification of specific interacting glycoproteins in plasma.</dcterms:abstract>
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    <dcterms:bibliographicCitation>First publ. in: Journal of Peptide Science 13 (2007), 12, pp. 803-810</dcterms:bibliographicCitation>
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    <dc:contributor>Bernevic, Bogdan</dc:contributor>
    <dc:creator>Przybylski, Michael</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-22T17:54:56Z</dc:date>
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    <dc:creator>Marquardt, Andreas</dc:creator>
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    <dc:creator>Bernevic, Bogdan</dc:creator>
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    <dc:language>eng</dc:language>
    <dc:contributor>Marquardt, Andreas</dc:contributor>
    <dcterms:issued>2007</dcterms:issued>
    <dc:contributor>Przybylski, Michael</dc:contributor>
    <dcterms:title>Identification, affinity characterisation and biological interactions of lectin-like peptide-carbohydrate complexes derived from human TNF-alpha using high-resolution mass spectrometry</dcterms:title>
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