Aufgrund von Vorbereitungen auf eine neue Version von KOPS, können am Montag, 6.2. und Dienstag, 7.2. keine Publikationen eingereicht werden. (Due to preparations for a new version of KOPS, no publications can be submitted on Monday, Feb. 6 and Tuesday, Feb. 7.)
Type of Publication: | Journal article |
Author: | Drescher, Malte; Godschalk, Frans; Veldhuis, Gertjan; Rooijen, Bart D. van; Subramaniam, Vinod; Huber, Martina |
Year of publication: | 2008 |
Published in: | ChemBioChem ; 9 (2008), 15. - pp. 2411-2416. - ISSN 1439-4227. - eISSN 1439-7633 |
Pubmed ID: | 18821550 |
DOI (citable link): | https://dx.doi.org/10.1002/cbic.200800238 |
Summary: |
The putative function of the Parkinson's disease-related protein -Synuclein (S) is thought to involve membrane binding. Therefore, the interaction of S with membranes composed of zwitterionic (POPC) and anionic (POPG) lipids was investigated through the mobility of spin labels attached to the protein. Differently labelled variants of S were produced, containing a spin label at positions 9, 18 (both helix 1), 69, 90 (both helix 2), and 140 (C terminus). Protein binding to POPC/POPG vesicles for all but S140 resulted in two mobility components with correlation times of 0.5 and 3 ns, for POPG mole fractions >0.4. Monitoring these components as a function of the POPG mole fraction revealed that at low negative-charge densities helix 1 is more tightly bound than helix 2; this indicates a partially bound form of S. Thus, the interaction of S with membranes of low charge densities might be initiated at helix 1. The local binding information thus obtained gives a more differentiated picture of the affinity of S to membranes. These findings contribute to our understanding of the details and structural consequences of S-membrane interactions.
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Subject (DDC): | 540 Chemistry |
Keywords: | EPR spectroscopy, membrane binding, site-directed spin labeling, synuclein |
Bibliography of Konstanz: | Yes |
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DRESCHER, Malte, Frans GODSCHALK, Gertjan VELDHUIS, Bart D. van ROOIJEN, Vinod SUBRAMANIAM, Martina HUBER, 2008. Spin-Label EPR on alpha-Synuclein Reveals Differences in the Membrane Binding Affinity of the Two Antiparallel Helices. In: ChemBioChem. 9(15), pp. 2411-2416. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.200800238
@article{Drescher2008SpinL-1043, title={Spin-Label EPR on alpha-Synuclein Reveals Differences in the Membrane Binding Affinity of the Two Antiparallel Helices}, year={2008}, doi={10.1002/cbic.200800238}, number={15}, volume={9}, issn={1439-4227}, journal={ChemBioChem}, pages={2411--2416}, author={Drescher, Malte and Godschalk, Frans and Veldhuis, Gertjan and Rooijen, Bart D. van and Subramaniam, Vinod and Huber, Martina} }