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Enhanced electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry of long-chain polysaccharides

Enhanced electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry of long-chain polysaccharides

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PERDIVARA, Irina, Eugen SISU, Ioana SISU, Nicolae DINCA, Kenneth B. TOMER, Michael PRZYBYLSKI, Alina D. ZAMFIR, 2008. Enhanced electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry of long-chain polysaccharides. In: Rapid Communications in Mass Spectrometry. 22(6), pp. 773-782. ISSN 0951-4198. eISSN 1097-0231

@article{Perdivara2008Enhan-1034, title={Enhanced electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry of long-chain polysaccharides}, year={2008}, number={6}, volume={22}, issn={0951-4198}, journal={Rapid Communications in Mass Spectrometry}, pages={773--782}, author={Perdivara, Irina and Sisu, Eugen and Sisu, Ioana and Dinca, Nicolae and Tomer, Kenneth B. and Przybylski, Michael and Zamfir, Alina D.} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/1034"> <dc:creator>Perdivara, Irina</dc:creator> <dc:creator>Przybylski, Michael</dc:creator> <dc:contributor>Perdivara, Irina</dc:contributor> <dcterms:abstract xml:lang="eng">A novel strategy was developed to extend the application of electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) to the analysis of long-chain polysaccharides. High molecular weight polydisperse maltodextrins (poly-(1-4) glucose) and dextrans (poly-(1-6) glucose) were chosen as model compounds in the present study. Increased ionization efficiency of these mixtures in the positive ion mode was achieved upon modification of their reducing end with nitrogen-containing groups. The derivatization method is based on the formation of a new CN bond between 1,6-hexamethylenediamine (HMD) and the reducing end of the polysaccharide, which exists in solution as an equilibrium between the hemiacetal and the open-ring aldehyde form. To achieve the chemical modification of the reducing end, two synthetic pathways were developed: (i) coupling of HMD by reductive amination and (ii) oxidation of the hemiacetal to lactone, followed by ring opening by HMD to yield the maltodextrin lactonamide of 1,6-hexanediamine (HMMD). Amino-functionalized polysaccharides were analyzed by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI FTICR-MS) in the positive ion mode by direct flow injection. The hexamethylenediamine (HMD) and maltodextrin lactonamide of 1,6-hexanediamine (HMMD) moieties provide increased proton affinities which dramatically improve the detection of the long-chain polysaccharides by FTICR-MS. The present approach allowed for identification of single components in mixtures with prominent heterogeneity in the degree of polymerization (DP), without the need for chromatographic separation prior to MS. The high mass accuracy was essential for the unambiguous characterization of the species observed in the analyzed mixtures. Furthermore, molecular components containing up to 42 glucose residues were detected, representing the largest polysaccharide chains analyzed so far by ESI FTICR-MS.</dcterms:abstract> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1034"/> <dcterms:issued>2008</dcterms:issued> <dc:contributor>Przybylski, Michael</dc:contributor> <dc:creator>Tomer, Kenneth B.</dc:creator> <dc:creator>Zamfir, Alina D.</dc:creator> <dc:contributor>Sisu, Ioana</dc:contributor> <dc:creator>Sisu, Ioana</dc:creator> <dcterms:title>Enhanced electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry of long-chain polysaccharides</dcterms:title> <dc:contributor>Tomer, Kenneth B.</dc:contributor> <dc:contributor>Dinca, Nicolae</dc:contributor> <dc:language>eng</dc:language> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-22T17:54:52Z</dcterms:available> <dc:creator>Dinca, Nicolae</dc:creator> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:contributor>Sisu, Eugen</dc:contributor> <dc:rights>deposit-license</dc:rights> <dcterms:bibliographicCitation>First publ. in: Rapid Communications in Mass Spectrometry 22 (2008), 6, pp. 773-782</dcterms:bibliographicCitation> <dc:contributor>Zamfir, Alina D.</dc:contributor> <dc:creator>Sisu, Eugen</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-22T17:54:52Z</dc:date> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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