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Identification of the molecular composition of the 20S proteasome of mouse intestine by high-resolution mass spectrometric proteome analysis

Identification of the molecular composition of the 20S proteasome of mouse intestine by high-resolution mass spectrometric proteome analysis

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WEBER, Reinhold, Regina PREYWISCH, Nikolay YOUHNOVSKI, Marcus GROETTRUP, Michael PRZYBYLSKI, 2009. Identification of the molecular composition of the 20S proteasome of mouse intestine by high-resolution mass spectrometric proteome analysis. In: Methods in Molecular Biology. 564, pp. 173-186. ISSN 1064-3745

@article{Weber2009Ident-10054, title={Identification of the molecular composition of the 20S proteasome of mouse intestine by high-resolution mass spectrometric proteome analysis}, year={2009}, doi={10.1007/978-1-60761-157-8_10}, volume={564}, issn={1064-3745}, journal={Methods in Molecular Biology}, pages={173--186}, author={Weber, Reinhold and Preywisch, Regina and Youhnovski, Nikolay and Groettrup, Marcus and Przybylski, Michael} }

<rdf:RDF xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:dcterms="http://purl.org/dc/terms/" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/10054"> <dc:contributor>Weber, Reinhold</dc:contributor> <dcterms:abstract xml:lang="eng">In the last years, intracellular protein degradation by the proteasome has become a focus area of scientific interest. Here, we describe a proteomics approach for the molecular mapping of the constituents of the proteolytically active core particle, the constitutive 20S proteasome from mouse intestine. In addition to the proteomics workflow widely used for protein isolation, gel electrophoretic separation, in-gel digestion, and UV-MALDI mass spectrometry, high-resolution Fourier transform ion cyclotron resonance mass spectrometry using infrared-MALDI ionisation (IR-MALDI FTICR-MS) has been employed as an efficient method for protein identification by peptide mass fingerprint. The 20S proteasome subunits α1 α7 and β1 β7 were completely and unambiguously identified. In addition to subunits β1 and β2, the corresponding inducible subunits being part of the immuno-proteasome were identified. The subunit β5i was found to completely replace the corresponding constitutive subunit, suggesting a high proteolytic activity of the intestinal proteasome leading to increased production of antigenic peptides. The high mass accuracy in the low ppm range and resolution of FTICR-MS provide direct identifications of individual proteins as mixtures such as components resulting from incomplete electrophoretic separation. In addition, the comparison of UV- and IR-MALDI FTICR-MS may provide details of fragmentation and rearrangement reactions that may occur under UV-MALDI ionisation conditions.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:16:16Z</dc:date> <dcterms:bibliographicCitation>First publ. in: Methods in Molecular Biology ; 564 (2009). - S. 173-186</dcterms:bibliographicCitation> <dcterms:issued>2009</dcterms:issued> <dc:rights>deposit-license</dc:rights> <dc:contributor>Przybylski, Michael</dc:contributor> <dc:format>application/pdf</dc:format> <dcterms:rights rdf:resource="http://nbn-resolving.org/urn:nbn:de:bsz:352-20140905103416863-3868037-7"/> <dc:creator>Weber, Reinhold</dc:creator> <dc:contributor>Youhnovski, Nikolay</dc:contributor> <dc:creator>Groettrup, Marcus</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/10054"/> <dc:creator>Przybylski, Michael</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Groettrup, Marcus</dc:contributor> <dcterms:title>Identification of the molecular composition of the 20S proteasome of mouse intestine by high-resolution mass spectrometric proteome analysis</dcterms:title> <dc:creator>Youhnovski, Nikolay</dc:creator> <dc:contributor>Preywisch, Regina</dc:contributor> <dc:creator>Preywisch, Regina</dc:creator> </rdf:Description> </rdf:RDF>

Dateiabrufe seit 01.10.2014 (Informationen über die Zugriffsstatistik)

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