Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP

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dc.contributor.authorNeuhaus, Klaus
dc.contributor.authorLandstorfer, Richard
dc.contributor.authorSimon, Svenja
dc.contributor.authorSchober, Steffen
dc.contributor.authorWright, Patrick R.
dc.contributor.authorSmith, Cameron
dc.contributor.authorBackofen, Rolf
dc.contributor.authorWecko, Romy
dc.contributor.authorKeim, Daniel A.
dc.contributor.authorScherer, Siegfried
dc.date.accessioned2017-05-11T08:30:13Z
dc.date.available2017-05-11T08:30:13Z
dc.date.issued2017eng
dc.description.abstractWhile NGS allows rapid global detection of transcripts, it remains difficult to distinguish ncRNAs from short mRNAs. To detect potentially translated RNAs, we developed an improved protocol for bacterial ribosomal footprinting (RIBOseq). This allowed distinguishing ncRNA from mRNA in EHEC. A high ratio of ribosomal footprints per transcript (ribosomal coverage value, RCV) is expected to indicate a translated RNA, while a low RCV should point to a non-translated RNA.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1186/s12864-017-3586-9eng
dc.identifier.pmid28245801eng
dc.identifier.ppn488503493
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/38838
dc.language.isoengeng
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.ddc004eng
dc.titleDifferentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhPeng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Neuhaus2017Diffe-38838,
  year={2017},
  doi={10.1186/s12864-017-3586-9},
  title={Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP},
  number={1},
  volume={18},
  journal={BMC Genomics},
  author={Neuhaus, Klaus and Landstorfer, Richard and Simon, Svenja and Schober, Steffen and Wright, Patrick R. and Smith, Cameron and Backofen, Rolf and Wecko, Romy and Keim, Daniel A. and Scherer, Siegfried},
  note={Article Number: 216}
}
kops.citation.iso690NEUHAUS, Klaus, Richard LANDSTORFER, Svenja SIMON, Steffen SCHOBER, Patrick R. WRIGHT, Cameron SMITH, Rolf BACKOFEN, Romy WECKO, Daniel A. KEIM, Siegfried SCHERER, 2017. Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP. In: BMC Genomics. 2017, 18(1), 216. eISSN 1471-2164. Available under: doi: 10.1186/s12864-017-3586-9deu
kops.citation.iso690NEUHAUS, Klaus, Richard LANDSTORFER, Svenja SIMON, Steffen SCHOBER, Patrick R. WRIGHT, Cameron SMITH, Rolf BACKOFEN, Romy WECKO, Daniel A. KEIM, Siegfried SCHERER, 2017. Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP. In: BMC Genomics. 2017, 18(1), 216. eISSN 1471-2164. Available under: doi: 10.1186/s12864-017-3586-9eng
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