Publikation:

An artificial PAP gene breaks self-tolerance and promotes tumor regression in the TRAMP model for prostate carcinoma

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2012

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Reichardt, Wilfried

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http://nbn-resolving.de/urn:nbn:de:bsz:352-180353
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https://doi.org/10.1038/mt.2011.241
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Molecular Therapy. 2012, 20(3), pp. 555-564. ISSN 1525-0016. eISSN 1525-0024. Available under: doi: 10.1038/mt.2011.241

Zusammenfassung

Prostate cancer (PCa) is the most commonly diagnosed type of cancer in men in western industrialized countries. As a public health burden, the need for the invention of new cost-saving PCa immunotherapies is apparent. In this study, we present a DNA vaccine encoding for the prostate-specific antigen prostatic acid phosphatase (PAP) linked to the J-domain and the SV40 enhancer sequence. The PAP DNA vaccine induced a strong PAP-specific cellular immune response after electroporation (EP)-based delivery in C57BL/6 mice. Splenocytes from mice immunized with PAP recognized the naturally processed PAP epitopes, indicating that vaccination with the PAP-J gene broke its self-tolerance against PAP. Remarkably, DNA vaccination with PAP-J inhibited tumor growth in the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) mouse model that closely resembled human PCa. Therefore, this study highlights a novel cancer immunotherapy approach with the potential to control PCa in clinical settings.

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570 Biowissenschaften, Biologie

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ISO 690SPIES, Elmar, Wilfried REICHARDT, Gerardo Omar ALVAREZ SALINAS, Marcus GRÖTTRUP, Peter ÖHLSCHLÄGER, 2012. An artificial PAP gene breaks self-tolerance and promotes tumor regression in the TRAMP model for prostate carcinoma. In: Molecular Therapy. 2012, 20(3), pp. 555-564. ISSN 1525-0016. eISSN 1525-0024. Available under: doi: 10.1038/mt.2011.241
BibTex
@article{Spies2012-03artif-18035,
  year={2012},
  doi={10.1038/mt.2011.241},
  title={An artificial PAP gene breaks self-tolerance and promotes tumor regression in the TRAMP model for prostate carcinoma},
  number={3},
  volume={20},
  issn={1525-0016},
  journal={Molecular Therapy},
  pages={555--564},
  author={Spies, Elmar and Reichardt, Wilfried and Alvarez Salinas, Gerardo Omar and Gröttrup, Marcus and Öhlschläger, Peter}
}
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