Publikation: Pterin-4a-carbinolamine dehydratase from pseudomonas aeruginosa : characterization, catalytic mechanism and comparison to the human enzyme
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be involved in dehydration catalysis. The three-dimensional positions of these residues match those of corresponding histidines at the active center of human PCD. Based on the coincidence of catalytic parameters, and on the similar effects induced by the mutations, it is concluded that the substrate binding mode and the reaction mechanisms of bacterial and human PCD are basically identical.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
KÖSTER, Sandra, Gunter STIER, Niels KUBASCH, Hans-Christoph CURTIUS, Sandro GHISLA, 1998. Pterin-4a-carbinolamine dehydratase from pseudomonas aeruginosa : characterization, catalytic mechanism and comparison to the human enzyme. In: Biological Chemistry. 1998, 379(12), pp. 1427-1432. ISSN 1431-6730. eISSN 1437-4315. Available under: doi: 10.1515/bchm.1998.379.12.1427BibTex
@article{Koster1998Pteri-8433, year={1998}, doi={10.1515/bchm.1998.379.12.1427}, title={Pterin-4a-carbinolamine dehydratase from pseudomonas aeruginosa : characterization, catalytic mechanism and comparison to the human enzyme}, number={12}, volume={379}, issn={1431-6730}, journal={Biological Chemistry}, pages={1427--1432}, author={Köster, Sandra and Stier, Gunter and Kubasch, Niels and Curtius, Hans-Christoph and Ghisla, Sandro} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8433"> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8433/1/Pterin_4a_carbinolamine_dehydratase_from_pseudomonas_aeruginosa.pdf"/> <dcterms:bibliographicCitation>First publ. in: Biological Chemistry 379 (1998), pp. 1427-1432</dcterms:bibliographicCitation> <dcterms:title>Pterin-4a-carbinolamine dehydratase from pseudomonas aeruginosa : characterization, catalytic mechanism and comparison to the human enzyme</dcterms:title> <dc:contributor>Ghisla, Sandro</dc:contributor> <dc:creator>Köster, Sandra</dc:creator> <dc:contributor>Stier, Gunter</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Curtius, Hans-Christoph</dc:contributor> <dc:creator>Curtius, Hans-Christoph</dc:creator> <dc:contributor>Köster, Sandra</dc:contributor> <dc:format>application/pdf</dc:format> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8433"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8433/1/Pterin_4a_carbinolamine_dehydratase_from_pseudomonas_aeruginosa.pdf"/> <dc:creator>Ghisla, Sandro</dc:creator> <dc:language>eng</dc:language> <dcterms:issued>1998</dcterms:issued> <dc:creator>Stier, Gunter</dc:creator> <dcterms:abstract xml:lang="eng">The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be involved in dehydration catalysis. The three-dimensional positions of these residues match those of corresponding histidines at the active center of human PCD. Based on the coincidence of catalytic parameters, and on the similar effects induced by the mutations, it is concluded that the substrate binding mode and the reaction mechanisms of bacterial and human PCD are basically identical.</dcterms:abstract> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dc:creator>Kubasch, Niels</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:35Z</dc:date> <dc:contributor>Kubasch, Niels</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:35Z</dcterms:available> </rdf:Description> </rdf:RDF>