Publikation:

Pterin-4a-carbinolamine dehydratase from pseudomonas aeruginosa : characterization, catalytic mechanism and comparison to the human enzyme

Lade...
Vorschaubild

Datum

1998

Autor:innen

Köster, Sandra
Stier, Gunter
Kubasch, Niels
Curtius, Hans-Christoph

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Biological Chemistry. 1998, 379(12), pp. 1427-1432. ISSN 1431-6730. eISSN 1437-4315. Available under: doi: 10.1515/bchm.1998.379.12.1427

Zusammenfassung

The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be involved in dehydration catalysis. The three-dimensional positions of these residues match those of corresponding histidines at the active center of human PCD. Based on the coincidence of catalytic parameters, and on the similar effects induced by the mutations, it is concluded that the substrate binding mode and the reaction mechanisms of bacterial and human PCD are basically identical.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

DCoH, Dehydratase, Phenylalanine hydroxylase, Tetrahydrobiopterin

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690KÖSTER, Sandra, Gunter STIER, Niels KUBASCH, Hans-Christoph CURTIUS, Sandro GHISLA, 1998. Pterin-4a-carbinolamine dehydratase from pseudomonas aeruginosa : characterization, catalytic mechanism and comparison to the human enzyme. In: Biological Chemistry. 1998, 379(12), pp. 1427-1432. ISSN 1431-6730. eISSN 1437-4315. Available under: doi: 10.1515/bchm.1998.379.12.1427
BibTex
@article{Koster1998Pteri-8433,
  year={1998},
  doi={10.1515/bchm.1998.379.12.1427},
  title={Pterin-4a-carbinolamine dehydratase from pseudomonas aeruginosa : characterization, catalytic mechanism and comparison to the human enzyme},
  number={12},
  volume={379},
  issn={1431-6730},
  journal={Biological Chemistry},
  pages={1427--1432},
  author={Köster, Sandra and Stier, Gunter and Kubasch, Niels and Curtius, Hans-Christoph and Ghisla, Sandro}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8433">
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8433/1/Pterin_4a_carbinolamine_dehydratase_from_pseudomonas_aeruginosa.pdf"/>
    <dcterms:bibliographicCitation>First publ. in: Biological Chemistry 379 (1998), pp. 1427-1432</dcterms:bibliographicCitation>
    <dcterms:title>Pterin-4a-carbinolamine dehydratase from pseudomonas aeruginosa : characterization, catalytic mechanism and comparison to the human enzyme</dcterms:title>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
    <dc:creator>Köster, Sandra</dc:creator>
    <dc:contributor>Stier, Gunter</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Curtius, Hans-Christoph</dc:contributor>
    <dc:creator>Curtius, Hans-Christoph</dc:creator>
    <dc:contributor>Köster, Sandra</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8433"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8433/1/Pterin_4a_carbinolamine_dehydratase_from_pseudomonas_aeruginosa.pdf"/>
    <dc:creator>Ghisla, Sandro</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:issued>1998</dcterms:issued>
    <dc:creator>Stier, Gunter</dc:creator>
    <dcterms:abstract xml:lang="eng">The three-dimensional structure of pterin-4a-carbinolamine dehydratase (PCD) from Pseudomonas aeruginosa has been solved. Based on this we have investigated the roles of putative active center residues through functional replacement by site-directed mutagenesis. Three histidines, His73, His74 and His91, appear to be involved in dehydration catalysis. The three-dimensional positions of these residues match those of corresponding histidines at the active center of human PCD. Based on the coincidence of catalytic parameters, and on the similar effects induced by the mutations, it is concluded that the substrate binding mode and the reaction mechanisms of bacterial and human PCD are basically identical.</dcterms:abstract>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dc:creator>Kubasch, Niels</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:35Z</dc:date>
    <dc:contributor>Kubasch, Niels</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:43:35Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen