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Nonlinear electron paramagnetic resonance studies of the interaction of cytochrome c oxidase with spin-labeled lipids in gel-phase membranes

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2000

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Páli, Tibor
Powell, Gary L.
Marsh, Derek

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Biochemistry. 2000, 39(9), pp. 2355-2361. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi992478p

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The interaction of lipids, spin-labeled at different positions in the sn-2 chain, with cytochromec oxidase reconstituted in gel-phase membranes of dimyristoylphosphatidylglycerol has been studied by electron paramagnetic resonance (EPR) spectroscopy. Nonlinear EPR methods, both saturation transferEPR and progressive saturation EPR, were used. Interaction with the protein largely removes the flexibility gradient of the lipid chains in gel-phase membranes. The rotational mobility of the chain segments is reduced, relative to that for gel-phase lipids, by the intramembranous interaction with cytochrome c oxidase.
This holds for all positions of chain labeling, but the relative effect is greater for chain segments closer to the terminal methyl ends. Modification of the paramagnetic metal-ion centers in the protein by binding azide has a pronounced effect on the spin-lattice relaxation of the lipid spin labels. This demonstrates that the centers modified are sufficiently close to the first-shell lipids to give appreciable dipolar interactions and that their vertical location in the membrane is closer to the 5-position than to the 14-position of the lipid chains.

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570 Biowissenschaften, Biologie

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ISO 690PÁLI, Tibor, Jörg KLEINSCHMIDT, Gary L. POWELL, Derek MARSH, 2000. Nonlinear electron paramagnetic resonance studies of the interaction of cytochrome c oxidase with spin-labeled lipids in gel-phase membranes. In: Biochemistry. 2000, 39(9), pp. 2355-2361. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi992478p
BibTex
@article{Pali2000Nonli-6863,
  year={2000},
  doi={10.1021/bi992478p},
  title={Nonlinear electron paramagnetic resonance studies of the interaction of cytochrome c oxidase with spin-labeled lipids in gel-phase membranes},
  number={9},
  volume={39},
  issn={0006-2960},
  journal={Biochemistry},
  pages={2355--2361},
  author={Páli, Tibor and Kleinschmidt, Jörg and Powell, Gary L. and Marsh, Derek}
}
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