Expanding the Genetic Code for Site-Directed Spin-Labeling

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Drescher_2-3ahh6n4nc9ro7.pdf(1.29 MB)
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2019
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urn:nbn:de:bsz:352-2-3ahh6n4nc9ro7
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10.3390/ijms20020373
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CC BY 4.0

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772027
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SPICE: Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes
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International journal of molecular sciences ; 20 (2019), 2. - 373. - eISSN 1422-0067
Abstract
Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy enables studies of the structure, dynamics, and interactions of proteins in the noncrystalline state. The scope and analytical value of SDSL⁻EPR experiments crucially depends on the employed labeling strategy, with key aspects being labeling chemoselectivity and biocompatibility, as well as stability and spectroscopic properties of the resulting label. The use of genetically encoded noncanonical amino acids (ncAA) is an emerging strategy for SDSL that holds great promise for providing excellent chemoselectivity and potential for experiments in complex biological environments such as living cells. We here give a focused overview of recent advancements in this field and discuss their potentials and challenges for advancing SDSL⁻EPR studies.
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540 Chemistry
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noncanonical amino acids; bioorthogonal chemistry; spin labeling; protein conformation; EPR spectroscopy; macromolecular dynamics
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ISO 690BRAUN, Theresa S., Malte DRESCHER, Daniel SUMMERER, 2019. Expanding the Genetic Code for Site-Directed Spin-Labeling. In: International journal of molecular sciences. 20(2), 373. eISSN 1422-0067. Available under: doi: 10.3390/ijms20020373
BibTex
@article{Braun2019-01-16Expan-45319,
  year={2019},
  doi={10.3390/ijms20020373},
  title={Expanding the Genetic Code for Site-Directed Spin-Labeling},
  number={2},
  volume={20},
  journal={International journal of molecular sciences},
  author={Braun, Theresa S. and Drescher, Malte and Summerer, Daniel},
  note={Correction: https://doi.org/10.3390/ijms22041574 Article Number: 373}
}
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Correction: https://doi.org/10.3390/ijms22041574
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