Publikation: Expanding the Genetic Code for Site-Directed Spin-Labeling
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Datum
2019
Herausgeber:innen
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European Union (EU): 772027
Projekt
SPICE: Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes
Open Access-Veröffentlichung
Open Access Gold
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Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
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Zeitschriftenartikel
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Published
Erschienen in
International journal of molecular sciences. 2019, 20(2), 373. eISSN 1422-0067. Available under: doi: 10.3390/ijms20020373
Zusammenfassung
Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy enables studies of the structure, dynamics, and interactions of proteins in the noncrystalline state. The scope and analytical value of SDSL⁻EPR experiments crucially depends on the employed labeling strategy, with key aspects being labeling chemoselectivity and biocompatibility, as well as stability and spectroscopic properties of the resulting label. The use of genetically encoded noncanonical amino acids (ncAA) is an emerging strategy for SDSL that holds great promise for providing excellent chemoselectivity and potential for experiments in complex biological environments such as living cells. We here give a focused overview of recent advancements in this field and discuss their potentials and challenges for advancing SDSL⁻EPR studies.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
noncanonical amino acids; bioorthogonal chemistry; spin labeling; protein conformation; EPR spectroscopy; macromolecular dynamics
Konferenz
Rezension
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ISO 690
BRAUN, Theresa S., Malte DRESCHER, Daniel SUMMERER, 2019. Expanding the Genetic Code for Site-Directed Spin-Labeling. In: International journal of molecular sciences. 2019, 20(2), 373. eISSN 1422-0067. Available under: doi: 10.3390/ijms20020373BibTex
@article{Braun2019-01-16Expan-45319,
year={2019},
doi={10.3390/ijms20020373},
title={Expanding the Genetic Code for Site-Directed Spin-Labeling},
number={2},
volume={20},
journal={International journal of molecular sciences},
author={Braun, Theresa S. and Drescher, Malte and Summerer, Daniel},
note={Correction: https://doi.org/10.3390/ijms22041574 Article Number: 373}
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Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Correction: https://doi.org/10.3390/ijms22041574
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
