Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10

Aichem, Annette; Boehm, Annika N.; Catone, Nicola; Schmidtke, Gunter; Gröttrup, Marcus

Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2019

Autor:innen

Aichem, Annette

Boehm, Annika N.

Catone, Nicola

Schmidtke, Gunter

Gröttrup, Marcus

DOI (zitierfähiger Link)

https://doi.org/10.1016/bs.mie.2018.12.040

Sammlungen

Biologie

Publikationstyp

Beitrag zu einem Sammelband

Publikationsstatus

Published

Erschienen in

HOCHSTRASSER, Mark, ed.. Ubiquitin and Ubiquitin-like Protein Modifiers. Cambridge, MA: Academic Press, 2019, pp. 229-256. ISBN 978-0-12-816359-7. Available under: doi: 10.1016/bs.mie.2018.12.040

Zusammenfassung

The ubiquitin-like modifier FAT10 (also called ubiquitin D (UBD)) interacts noncovalently with a substantial number of proteins and also gets covalently conjugated to many substrate proteins, leading to their degradation by the 26S proteasome. FAT10 comprises two loosely folded ubiquitin-like domains that are connected by a flexible linker, and this unusual structure makes it highly prone to aggregation. Here, we report methods to purify high amounts of soluble recombinant FAT10 for various uses, such as in vitro FAT10ylation assays. In addition, we describe how to generate and handle overexpressed as well as endogenous FAT10 in cellulo for use in immunoprecipitations, Western blot analyses, and FAT10 degradation studies.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

AICHEM, Annette, Annika N. BOEHM, Nicola CATONE, Gunter SCHMIDTKE, Marcus GRÖTTRUP, 2019. Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10. In: HOCHSTRASSER, Mark, ed.. Ubiquitin and Ubiquitin-like Protein Modifiers. Cambridge, MA: Academic Press, 2019, pp. 229-256. ISBN 978-0-12-816359-7. Available under: doi: 10.1016/bs.mie.2018.12.040

BibTex

@incollection{Aichem2019Analy-45522,
  year={2019},
  doi={10.1016/bs.mie.2018.12.040},
  title={Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10},
  number={618},
  isbn={978-0-12-816359-7},
  publisher={Academic Press},
  address={Cambridge, MA},
  series={Methods in Enzymology},
  booktitle={Ubiquitin and Ubiquitin-like Protein Modifiers},
  pages={229--256},
  editor={Hochstrasser, Mark},
  author={Aichem, Annette and Boehm, Annika N. and Catone, Nicola and Schmidtke, Gunter and Gröttrup, Marcus}
}

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Universitätsbibliographie

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