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Protein-DNA interactions at the simian virus 40 origin of replication

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1988

Autor:innen

Baur, Claus-Peter
Klausing, Kay
Stahl, Hans

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https://doi.org/10.1016/0167-4781(88)90111-X
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Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1988, 951(2-3), pp. 388-395. ISSN 0167-4781. eISSN 1879-2634. Available under: doi: 10.1016/0167-4781(88)90111-X

Zusammenfassung

Simian Virus 40 (SV40)-encoded large T antigen has an intrinsic ATP-dependent DNA-unwinding activity which is necessary for an early step in the activation of the viral origin of replication. Isolated T antigen unwinds any double-stranded DNA, regardless of whether it is linear or circularly closed. However, initiation of DNA replication depends on an intact origin of replication, and even minor deviations from the wild-type origin sequence abolish the template activity of an origin-bearing plasmid. This discrepancy suggests that T antigen may not be sufficient for origin activation and that other, probably cellular, functions are involved. We have isolated a cellular protein, the LOB protein, which specifically interacts with the AT-rich region of the SV40 origin and which induces a pronounced bending of the bound DNA.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Protein-DNA interaction; DNA replication; T antigen; (SV40)

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ISO 690BAUR, Claus-Peter, Kay KLAUSING, Martin SCHEFFNER, Hans STAHL, Rolf KNIPPERS, 1988. Protein-DNA interactions at the simian virus 40 origin of replication. In: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1988, 951(2-3), pp. 388-395. ISSN 0167-4781. eISSN 1879-2634. Available under: doi: 10.1016/0167-4781(88)90111-X
BibTex
@article{Baur1988Prote-42778,
  year={1988},
  doi={10.1016/0167-4781(88)90111-X},
  title={Protein-DNA interactions at the simian virus 40 origin of replication},
  number={2-3},
  volume={951},
  issn={0167-4781},
  journal={Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression},
  pages={388--395},
  author={Baur, Claus-Peter and Klausing, Kay and Scheffner, Martin and Stahl, Hans and Knippers, Rolf}
}
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