Publikation: Study of metal-containing proteins in the roots of Elsholtzia splendens using LA-ICP-MS and LC–tandem mass spectrometry
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2011
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International Journal of Mass Spectrometry. 2011, 307(1-3), pp. 85-91. ISSN 1387-3806. Available under: doi: 10.1016/j.ijms.2011.01.018
Zusammenfassung
In the present study, the metal-containing proteins in the roots of Cu-tolerant plant Elsholtzia splendens were investigated. The proteins from the plant roots were extracted and separated by two-dimensional gel electrophoresis (2D GE). Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was used to screen metal-containing proteins on the dried 2D gels. Images of Cu, Zn, Na, Mg, K, Ca, Mn, Fe, P and S, and also of Ag, were obtained by scanning the gel (line by line) using the LA-ICP-MS techniques developed in our laboratory. Cu-, Zn- and Mg-containing proteins were found in the gel sections of interest via LA-ICP-MS imaging. These protein spots were then in-gel digested with trypsin and analyzed by liquid chromatography tandem mass spectrometry (LC–MS/MS) for identification. The mechanisms involved in Cu tolerance and accumulation were also discussed.
Zusammenfassung in einer weiteren Sprache
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540 Chemie
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Elsholtzia splendens, imaging of 2D gels, laser ablation ICP-MS, LC–MS/MS, metal-containing proteins
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WU, Bei, Iuliana SUSNEA, Yingxu CHEN, Michael PRZYBYLSKI, J. Sabine BECKER, 2011. Study of metal-containing proteins in the roots of Elsholtzia splendens using LA-ICP-MS and LC–tandem mass spectrometry. In: International Journal of Mass Spectrometry. 2011, 307(1-3), pp. 85-91. ISSN 1387-3806. Available under: doi: 10.1016/j.ijms.2011.01.018BibTex
@article{Wu2011Study-16773,
year={2011},
doi={10.1016/j.ijms.2011.01.018},
title={Study of metal-containing proteins in the roots of Elsholtzia splendens using LA-ICP-MS and LC–tandem mass spectrometry},
number={1-3},
volume={307},
issn={1387-3806},
journal={International Journal of Mass Spectrometry},
pages={85--91},
author={Wu, Bei and Susnea, Iuliana and Chen, Yingxu and Przybylski, Michael and Becker, J. Sabine}
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<dcterms:abstract xml:lang="eng">In the present study, the metal-containing proteins in the roots of Cu-tolerant plant Elsholtzia splendens were investigated. The proteins from the plant roots were extracted and separated by two-dimensional gel electrophoresis (2D GE). Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was used to screen metal-containing proteins on the dried 2D gels. Images of Cu, Zn, Na, Mg, K, Ca, Mn, Fe, P and S, and also of Ag, were obtained by scanning the gel (line by line) using the LA-ICP-MS techniques developed in our laboratory. Cu-, Zn- and Mg-containing proteins were found in the gel sections of interest via LA-ICP-MS imaging. These protein spots were then in-gel digested with trypsin and analyzed by liquid chromatography tandem mass spectrometry (LC–MS/MS) for identification. The mechanisms involved in Cu tolerance and accumulation were also discussed.</dcterms:abstract>
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