Electronically preresonant stimulated Raman scattering microscopy in the visible

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2023
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Applied Physics Letters. American Institute of Physics (AIP). 2023, 123(16), 163701. ISSN 0003-6951. eISSN 1077-3118. Available under: doi: 10.1063/5.0171725
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We report an experimental scheme for stimulated Raman scattering (SRS) microscopy with excitation in the visible spectral region. This allows electronically preresonant (epr) SRS microscopy of a broad range of chromophores with sensitivities as low as 1 μM. Our experiment is based on two synchronously near-infrared pumped optical parametric oscillators (OPO). One of the outputs is modulated at a fourth of the repetition rate with a novel broadband electro-optical modulator. Using a combination of spectral focusing and tuning of the OPO, we show the recording of epr-SRS spectra over the whole range of molecular vibrations at a speed up to 20 times faster than classical wavelength tuning. The imaging capabilities of this setup are demonstrated with material scientific and cellular samples.

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ISO 690CHOORAKUTTIL, Ashwin J. X., Andrea PRUCCOLI, Martin WINTERHALDER, Peyman ZIRAK, Dominykas GUDAVIČIUS, Giedrius MARTYNAITIS, Dalius PETRULIONIS, Danielius SAMSONAS, Lukas KONTENIS, Andreas ZUMBUSCH, 2023. Electronically preresonant stimulated Raman scattering microscopy in the visible. In: Applied Physics Letters. American Institute of Physics (AIP). 2023, 123(16), 163701. ISSN 0003-6951. eISSN 1077-3118. Available under: doi: 10.1063/5.0171725
BibTex
@article{Choorakuttil2023Elect-68234,
  year={2023},
  doi={10.1063/5.0171725},
  title={Electronically preresonant stimulated Raman scattering microscopy in the visible},
  number={16},
  volume={123},
  issn={0003-6951},
  journal={Applied Physics Letters},
  author={Choorakuttil, Ashwin J. X. and Pruccoli, Andrea and Winterhalder, Martin and Zirak, Peyman and Gudavičius, Dominykas and Martynaitis, Giedrius and Petrulionis, Dalius and Samsonas, Danielius and Kontenis, Lukas and Zumbusch, Andreas},
  note={Article Number: 163701}
}
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