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Crystal structure of oxidized flavodoxin, an essential protein in Helicobacter pylori

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2002

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Freigang, Jörg
Schäfer, Klaus P.
Paul, Ralf

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Protein Science. 2002, 11(2), pp. 253-261. ISSN 0961-8368. eISSN 0961-8368. Available under: doi: 10.1110/ps.28602

Zusammenfassung

The redox protein flavodoxin has been shown earlier to be reduced by the pyruvate-oxidoreductase (POR) enzyme complex of Helicobacter pylori, and also was proposed to be involved in the pathogenesis of gastric mucosa-associated lymphoid-tissue lymphoma (MALToma). Here, we report its X-ray structure, which is similar to flavodoxins of other bacteria and cyanobacteria. However, H. pylori flavodoxin has an alanine residue near the isoalloxazine ring of its cofactor flavin mononucleotide (FMN), while the other previously crystallized flavodoxins have a larger hydrophobic residue at this position. This creates a solute filled hole near the FMN cofactor of H. pylori flavodoxin. We also show that flavodoxin is essential for the survival of H. pylori, and conclude that its structure can be used as a starting point for the modeling of an inhibitor for the interaction between the POR-enzyme complex and flavodoxin.

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570 Biowissenschaften, Biologie

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ISO 690FREIGANG, Jörg, Kay DIEDERICHS, Klaus P. SCHÄFER, Wolfram WELTE, Ralf PAUL, 2002. Crystal structure of oxidized flavodoxin, an essential protein in Helicobacter pylori. In: Protein Science. 2002, 11(2), pp. 253-261. ISSN 0961-8368. eISSN 0961-8368. Available under: doi: 10.1110/ps.28602
BibTex
@article{Freigang2002Cryst-8450,
  year={2002},
  doi={10.1110/ps.28602},
  title={Crystal structure of oxidized flavodoxin, an essential protein in Helicobacter pylori},
  number={2},
  volume={11},
  issn={0961-8368},
  journal={Protein Science},
  pages={253--261},
  author={Freigang, Jörg and Diederichs, Kay and Schäfer, Klaus P. and Welte, Wolfram and Paul, Ralf}
}
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