Publikation:

l-Selegiline Potentiates the Cellular Poly(ADP-Ribosyl)ationResponse to Ionizing Radiation

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2003

Autor:innen

Brabeck, Christine
Pfeiffer, Ragen
Leake, Alan
Beneke, Sascha
Meyer, Ralph

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Journal of Pharmacology and Experimental Therapeutics. American Society for Pharmacology and Experimental Therapeutics. 2003, 306(3), pp. 973-979. ISSN 0022-3565. eISSN 1521-0103. Available under: doi: 10.1124/jpet.103.051342

Zusammenfassung

DNA strand breaks induced by alkylating agents, oxidants, or ionizingradiation trigger the covalent modification of nuclear proteins withpoly(ADP-ribose), which is catalyzed for the most part by poly(ADP-ribose)polymerase-1 and plays a role in DNA base-excision repair.Poly(ADP-ribosyl)ation capacity of mononuclear blood cells correlatespositively with life span of mammalian species. Here, we show that l-selegiline, an anti-Parkinson drug with neuroprotective activityand life span-extending effect in laboratory animals, can potentiate γ-radiation-induced poly(ADP-ribose) formation in intact cells. COR4hamster cells were incubated with l-selegiline (50 nM) for varioustime periods, followed by γ-irradiation (45 Gy). Quantification ofcellular poly(ADP-ribose) levels at 10 min after starting the irradiationrevealed significant increases (up to 1.8-fold) in cells preincubated with thedrug for 8 h to 7 days compared with drug-free irradiated controls. There wasno selegiline-induced change in poly(ADP-ribose) levels of unirradiated cellsnor in basal or radiation-induced DNA strand breaks, respectively.Surprisingly, poly(ADP-ribose) polymerase-1 protein was down-regulated by l-selegiline treatment. Addition of l-selegiline topurified poly(ADP-ribose) polymerase-1 did not alter enzymatic activity. Inconclusion, the results of the present study identify a novel intervention topotentiate the cellular poly(ADP-ribosyl)ation response. We hypothesize thatthe effect of l-selegiline is due to modulation of accessoryproteins regulating poly(ADP-ribose) polymerase-1 activity and that increasedcellular poly- (ADP-ribosyl)ation capacity may contribute to theneuroprotective potential and/or life span extension afforded by l-selegiline.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690BRABECK, Christine, Ragen PFEIFFER, Alan LEAKE, Sascha BENEKE, Ralph MEYER, Alexander BÜRKLE, 2003. l-Selegiline Potentiates the Cellular Poly(ADP-Ribosyl)ationResponse to Ionizing Radiation. In: Journal of Pharmacology and Experimental Therapeutics. American Society for Pharmacology and Experimental Therapeutics. 2003, 306(3), pp. 973-979. ISSN 0022-3565. eISSN 1521-0103. Available under: doi: 10.1124/jpet.103.051342
BibTex
@article{Brabeck2003-09lSele-52313,
  year={2003},
  doi={10.1124/jpet.103.051342},
  title={l-Selegiline Potentiates the Cellular Poly(ADP-Ribosyl)ationResponse to Ionizing Radiation},
  number={3},
  volume={306},
  issn={0022-3565},
  journal={Journal of Pharmacology and Experimental Therapeutics},
  pages={973--979},
  author={Brabeck, Christine and Pfeiffer, Ragen and Leake, Alan and Beneke, Sascha and Meyer, Ralph and Bürkle, Alexander}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52313">
    <dc:creator>Bürkle, Alexander</dc:creator>
    <dc:contributor>Brabeck, Christine</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Meyer, Ralph</dc:creator>
    <dc:creator>Brabeck, Christine</dc:creator>
    <dc:language>eng</dc:language>
    <dc:creator>Leake, Alan</dc:creator>
    <dc:creator>Beneke, Sascha</dc:creator>
    <dc:contributor>Meyer, Ralph</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">DNA strand breaks induced by alkylating agents, oxidants, or ionizingradiation trigger the covalent modification of nuclear proteins withpoly(ADP-ribose), which is catalyzed for the most part by poly(ADP-ribose)polymerase-1 and plays a role in DNA base-excision repair.Poly(ADP-ribosyl)ation capacity of mononuclear blood cells correlatespositively with life span of mammalian species. Here, we show that l-selegiline, an anti-Parkinson drug with neuroprotective activityand life span-extending effect in laboratory animals, can potentiate γ-radiation-induced poly(ADP-ribose) formation in intact cells. COR4hamster cells were incubated with l-selegiline (50 nM) for varioustime periods, followed by γ-irradiation (45 Gy). Quantification ofcellular poly(ADP-ribose) levels at 10 min after starting the irradiationrevealed significant increases (up to 1.8-fold) in cells preincubated with thedrug for 8 h to 7 days compared with drug-free irradiated controls. There wasno selegiline-induced change in poly(ADP-ribose) levels of unirradiated cellsnor in basal or radiation-induced DNA strand breaks, respectively.Surprisingly, poly(ADP-ribose) polymerase-1 protein was down-regulated by l-selegiline treatment. Addition of l-selegiline topurified poly(ADP-ribose) polymerase-1 did not alter enzymatic activity. Inconclusion, the results of the present study identify a novel intervention topotentiate the cellular poly(ADP-ribosyl)ation response. We hypothesize thatthe effect of l-selegiline is due to modulation of accessoryproteins regulating poly(ADP-ribose) polymerase-1 activity and that increasedcellular poly- (ADP-ribosyl)ation capacity may contribute to theneuroprotective potential and/or life span extension afforded by l-selegiline.</dcterms:abstract>
    <dc:contributor>Leake, Alan</dc:contributor>
    <dc:contributor>Pfeiffer, Ragen</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-01-08T09:28:57Z</dcterms:available>
    <dcterms:issued>2003-09</dcterms:issued>
    <dc:contributor>Beneke, Sascha</dc:contributor>
    <dc:creator>Pfeiffer, Ragen</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/52313"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-01-08T09:28:57Z</dc:date>
    <dc:contributor>Bürkle, Alexander</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:title>l-Selegiline Potentiates the Cellular Poly(ADP-Ribosyl)ationResponse to Ionizing Radiation</dcterms:title>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:rights>terms-of-use</dc:rights>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Ja
Diese Publikation teilen