Publikation:

Studies with Old Yellow Enzyme, Reconstruction with Lumazine Analogs as Coenzyme

Lade...
Vorschaubild

Datum

1983

Autor:innen

Wetzel, Günter

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

DOI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Beitrag zu einem Sammelband
Publikationsstatus
Published

Erschienen in

BLAIR, John A., ed.. Chemistry and Biology of Pteridines. Berlin: de Gruyter, 1983, pp. 693-698

Zusammenfassung

8-Substituted lumazines can be regarded as "mutilated" flavins i.e. isoalloxazines lacking the benzene moiety. The 5'phosphorylated, 8-ribityl derivative binds to the apoenzyme of Old Yellow Enzyme with a Kd which is comparable to that of FMN (Kd 6x10-9 M, and 4x10-10 M for the analog, and FMN respectively). Binding induces profound spectral effects on the lumazine chromophore; this might be interpreted as reflecting a protein induced 7alpha-> 1 prototropy. The complex shows approx. 10% of the catalytic activity of native enzyme, in the oxidation of NADPH. Binding of aromatic molecules known to result in pronounced charge transfer spectra with native old yellow enzyme, have no spectral effect with the analog. These results indicate that the benzene moiety is important for the spectral effect, but not for catalysis.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690WETZEL, Günter, Sandro GHISLA, 1983. Studies with Old Yellow Enzyme, Reconstruction with Lumazine Analogs as Coenzyme. In: BLAIR, John A., ed.. Chemistry and Biology of Pteridines. Berlin: de Gruyter, 1983, pp. 693-698
BibTex
@incollection{Wetzel1983Studi-6580,
  year={1983},
  title={Studies with Old Yellow Enzyme, Reconstruction with Lumazine Analogs as Coenzyme},
  publisher={de Gruyter},
  address={Berlin},
  booktitle={Chemistry and Biology of Pteridines},
  pages={693--698},
  editor={Blair, John A.},
  author={Wetzel, Günter and Ghisla, Sandro}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6580">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:34Z</dcterms:available>
    <dc:format>application/pdf</dc:format>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6580/1/Studies_with_Old_Yellow_Enzyme_Reconstruction_with_Lumazine_Analogs_as_Coenzyme1.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:27:34Z</dc:date>
    <dc:creator>Ghisla, Sandro</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6580"/>
    <dcterms:bibliographicCitation>First publ. in: Chemistry and Biology of Pteridines / ed.: John A. Blair. Berlin : de Gruyter, 1983, pp. 693-698</dcterms:bibliographicCitation>
    <dc:creator>Wetzel, Günter</dc:creator>
    <dcterms:title>Studies with Old Yellow Enzyme, Reconstruction with Lumazine Analogs as Coenzyme</dcterms:title>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6580/1/Studies_with_Old_Yellow_Enzyme_Reconstruction_with_Lumazine_Analogs_as_Coenzyme1.pdf"/>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:contributor>Wetzel, Günter</dc:contributor>
    <dcterms:abstract xml:lang="eng">8-Substituted lumazines can be regarded as "mutilated" flavins i.e. isoalloxazines lacking the benzene moiety. The 5'phosphorylated, 8-ribityl derivative binds to the apoenzyme of Old Yellow Enzyme with a Kd which is comparable to that of FMN (Kd 6x10-9 M, and 4x10-10 M for the analog, and FMN respectively). Binding induces profound spectral effects on the lumazine chromophore; this might be interpreted as reflecting a protein induced 7alpha-&gt; 1 prototropy. The complex shows approx. 10% of the catalytic activity of native enzyme, in the oxidation of NADPH. Binding of aromatic molecules known to result in pronounced charge transfer spectra with native old yellow enzyme, have no spectral effect with the analog. These results indicate that the benzene moiety is important for the spectral effect, but not for catalysis.</dcterms:abstract>
    <dcterms:issued>1983</dcterms:issued>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen