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Caffeine-induced Ca2+ transients and exocytosis in paramecium cells : a correlated Ca2+ imaging and quenched-flow/freeze-fracture analysis

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1998

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Klauke, Norbert

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Journal of Membrane Biology. 1998, 161(1), pp. 65-81. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s002329900315

Zusammenfassung

Caffeine causes a [Ca2+]i increase in the cortex of Paramecium cells, followed by spillover with considerable attenuation, into central cell regions. From [Ca2+]resti 50 to 80 nm, [Ca2+]acti rises within ≤3 sec to 500 (trichocyst-free strain tl) or 220 nm (nondischarge strain nd9 28°C) in the cortex. Rapid confocal analysis of wildtype cells (7S) showed only a 2-fold cortical increase within 2 sec, accompanied by trichocyst exocytosis and a central Ca2+ spread during the subsequent ≥2 sec. Chelation of Ca2+o considerably attenuated [Ca2+]i increase. Therefore, caffeine may primarily mobilize cortical Ca2+ pools, superimposed by Ca2+ influx and spillover (particularly in tl cells with empty trichocyst docking sites). In nd cells, caffeine caused trichocyst contents to decondense internally (Ca2+-dependent stretching, normally occurring only after membrane fusion). With 7S cells this usually occurred only to a small extent, but with increasing frequency as [Ca2+]i signals were reduced by [Ca2+]ochelation. In this case, quenched-flow and ultrathin section or freeze-fracture analysis revealed dispersal of membrane components (without fusion) subsequent to internal contents decondensation, opposite to normal membrane fusion when a full [Ca2+]i signal was generated by caffeine stimulation (with Ca2+i and Ca2+o available). We conclude the following. (i) Caffeine can mobilize Ca2+ from cortical stores independent of the presence of Ca2+o. (ii) To yield adequate signals for normal exocytosis, Ca2+ release and Ca2+ influx both have to occur during caffeine stimulation. (iii) Insufficient [Ca2+]i increase entails caffeine-mediated access of Ca2+ to the secretory contents, thus causing their decondensation before membrane fusion can occur. (iv) Trichocyst decondensation in turn gives a signal for an unusual dissociation of docking/fusion components at the cell membrane. These observations imply different threshold [Ca2+]i-values for membrane fusion and contents discharge.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

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Caffeine, Calcium, Exocytosis, Paramecium, Secretion

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ISO 690KLAUKE, Norbert, Helmut PLATTNER, 1998. Caffeine-induced Ca2+ transients and exocytosis in paramecium cells : a correlated Ca2+ imaging and quenched-flow/freeze-fracture analysis. In: Journal of Membrane Biology. 1998, 161(1), pp. 65-81. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s002329900315
BibTex
@article{Klauke1998Caffe-7785,
  year={1998},
  doi={10.1007/s002329900315},
  title={Caffeine-induced Ca2+ transients and exocytosis in paramecium cells : a correlated Ca2+ imaging and quenched-flow/freeze-fracture analysis},
  number={1},
  volume={161},
  issn={0022-2631},
  journal={Journal of Membrane Biology},
  pages={65--81},
  author={Klauke, Norbert and Plattner, Helmut}
}
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